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271ANALESRANFwwwanalesranfcomMaternalfructoseintakemodulatescholesterolmetabolisminresponsetoaWesterndietintheoffspringPalomaLópezLaizElenaFausteetalAnRealAcadFarmVol88nº3(2022)·pp257-275RegardingtheMTTPhepaticexpressionthetrendtoanincreasealreadycommented fordescendantsreceiving fructosealone(Figure12) wasalso foundin theoffspring taking fructosepluscholesterolwithrespecttothecontrolgroupsregardlessthematernalintakeGiven that theproductof thisgeneisrelated to theassemblyoflipoproteinscontainingcholesterolandtriglyceridestheexpressionofthisgeneintheFChgroupisincreasedinaccordancewith2cHepaticMTTP,LDLRandSR-B1expressionThe Table8 shows statistically significantdifferencesin theexpressionofMTTPandLDLRgeneswithregardstothedietofthemaleoffspring(p=0015andp=0035respectively)InthecaseofSR-B1thisdifferenceisalmostsignificant(p=0082)ThusFigure16showsthehepaticgeneexpressionofMTTP,LDLRandSR-B1Figure15PlasmaHDLcholesterolandnon-HDLcholesterolconcentrationin themaleprogeny,after 21daysofreceivingdifferent treatments(bluebar water–Corangebar fructose–F,purplebar fructose+cholesterol-richdiet–FCh) Thebars thatpresentalightercolourcorrespond to thedescendantsofmothers thatweregivenwaterduringgestation(maternalcontrolgroup)andthebarswithadarkercolour,tothedescendantsofmothersthatdrankafructosesolutionduringgestation(maternalfructosegroup)Thedatapresentedaremeans±standarderror(SE)Thesymbol*denotesstatisticallysignificantdifferencesbetweendietsMTTPexpressionFPvalueη2MOTHER117202870033DIET475900150219MOTHER*DIET003909620002LDLRexpressionFPvalueη2MOTHER000109730000DIET369600350170MOTHER*DIET061205480033Table8 Two-way ANOVAofhepaticMTTP,LDLRand SR-B1expressionF SnedecorsF, whichisa statisticparameterbasedon theanalysisof varianceP valueprobabilityofobservingavaluewhichisatleastasextremeasthevalueobtainedfromthetest2proportionofthevariancethatisattributedtothemother,thedietortheinteractionbetweenboth Thegreencolourin thecellindicates that thedifferenceis statistically significant while yellowrepresents that thedifferenceisalmost statisticallysignificantTable8SR-B1expressionFPvalueη2MOTHER199201670052DIET268400820130MOTHER*DIET177201850090