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267ANALESRANFwwwanalesranfcomMaternalfructoseintakemodulatescholesterolmetabolisminresponsetoaWesterndietintheoffspringPalomaLópezLaizElenaFausteetalAnRealAcadFarmVol88nº3(2022)·pp257-275duetoboththematernaltreatment(M)andthediet(D)andinSRB1expressionduetotheinteractionbetweenbothtreatments(M*D)(LDLRp=0097M;p=0090D;SR-B1p=0052M*D)Thesepossiblestatisticaldifferenceswerefurtherstudiedthrough a posterioriBonferroni test and they canbe seeninFigure12ThehepaticexpressionofMTTPshowsatendencytoincreaseinmalesthatconsumedfructoseinbothgroupsofdescendants those fromcontrolmothersand fructose-mothers This trend41cHepaticMTTP,LDLRandSR-B1expressionBoth theMTTP,LDLRand SR-B1aregenescoding formoleculesinvolvedincholesterolmetabolismAfterperformingthetwo-wayANOVAwiththeexpressiondata for the threegenes thereisonlyonestatisticallysignificantdifference(Table4)intheexpressionofMTTPgenefortheinfluenceofthe diet(D)receivedby themalerats(p=0013)However, almost statisticallysignificantdifferenceswerefoundintheexpressionofLDLRMTTPexpressionFP valueη2MOTHER123102750034DIET489500130219MOTHER*DIET003709640002Table4LDLRexpressionFP valueη2MOTHER289500970074DIET258200900125MOTHER*DIET094303990050SR-B1expressionFP valueη2MOTHER000409500000DIET066505210036MOTHER*DIET322100520152Table4 Two-way ANOVAofhepaticMTTP,LDLRandSR-B1expressionFSnedecorsF,whichisastatisticparameterbasedon theanalysisof varianceP valueprobabilityof observinga value whichisatleastas extremeas the value obtained from the test 2proportion of the variance thatisattributed to themother, thediet or theinteractionbetweenbothThegreencolourinthecellindicatesthatthedifferenceisstatisticallysignificantwhileyellowrepresentsthatthedifferenceisalmoststatisticallysignificantFigure12MTTP,LDLRandSR-B1hepatic geneexpressionin themaleprogeny,after 21daysofreceivingdifferent treatments(bluebar water–Corangebar fructose–F,greenbartagatose–T)Thebarsthatpresentalightercolourcorrespondtothedescendantsofmothersthatweregivenwaterduringgestation(maternalcontrolroup)andthebarswithadarkercolour,tothedescendantsofmothersthatdrankafructosesolutionduringgestation(maternalfructosegroup)Thedatapresentedaremeans± standarderror(SE) The symbol#corresponds todifferences thatare statistically significantbetweenmaternalconsumptionand the symbol& todifferencesthatarealmoststatisticallysignificantbetweenmaternalintake