Page 93 - 87_01
P. 93
ANALES dure: characterization of size distribution, trapped volume and ability
RANF to maintain a membrane potential. Hope MJ, Bally MB, Webb G,
Cullis PR. Biochim Biophys Acta. 1985;812(1):55-65.
www.analesranf.com 14. Electric-field-dependent thermal fluctuations of giant vesicles. Mitov
MD, Méléard P, Winterhalter M, Angelova MI, Bothorel P. Phys Rev
destacar en particular a nuestro técnico de laboratorio, Dr. Jesús E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1993;
Sot, y a nuestra antigua colaboradora, ahora en Rabat, Dra. Hasna 48(1):628-631.
Ahyayauch. De los colegas internacionales que han compartido con 15. Giant unilamellar vesicles electroformed from native membranes
nosotros horas de análisis y discusión deseamos mencionar espe- and organic lipid mixtures under physiological conditions. Montes
cialmente a los profesores Kai Simons (Dresden), Dov Lichtenberg LR, Alonso A, Goñi FM, Bagatolli LA. Biophys J. 2007;
(Tel Aviv) y Heiko Heerklotz (Friburgo de Brisgovia). 93(10):3548-54.
16. Reconstitution of membrane processes. Racker E. Methods Enzymol.
Nuestras investigaciones han sido subvencionadas, a lo 1979; 55:699-711.
largo de los años, por la Unión Europea, el Gobierno de España, el 17. Special Issue: Detergents in biomembrane studies. Goñi FM, Alonso
Gobierno Vasco, la Diputación Foral de Bizkaia y la Universidad del A. (eds.). Biochim Biophys Acta. 2000; 1508:1-252.
País Vasco. 18. Liposomes as a model for solubilization and reconstitution of mem-
branes. Lichtenberg D. In: Handbook of Nonmedical Applications of
Conflicto de intereses Liposomes (Barenholz, Y. and Lasic, D.D., eds.), pp. 199–218,
Los autores no declaran conflicto de intereses de ningún 1996, CRC Press, Boca Raton, FL, USA.
19. Interactions of surfactants with lipid membranes. Heerklotz H. Q
tipo. Rev Biophys.2008; 41(3-4):205-64
20. Characterization of the solubilization of lipid bilayers by surfactants.
6. REFERENCIAS Lichtenberg D. Biochim Biophys Acta. 1985; 821(3):470-8.
21. Detergents: an overview. Linke D. Methods Enzymol. 2009; 463,
1. Surface and bulk interactions of lipids and water with a classification 603-617.
of biologically active lipids based on these interactions. Small DM. 22. Membrane proteins, lipids and detergents: not just a soap opera.
Fed Proc. 1970;29(4):1320-6. Seddon AM, Curnow P, Booth P.J. Biochim. Biophys. Acta 2004;
1666:105-117.
2. The hydrophobic effect and the organization of living matter. Tanford 23. Detergent-resistant membranes should not be identified with mem-
C. Science. 1978; 200(4345):1012-8. brane rafts. Lichtenberg D, Goñi FM, Heerklotz H. Trends Biochem
Sci. 2005; 30(8):430-6.
3. Solubilization of membranes by detergents. Helenius A, Simons K. 24. Membrane protein solubilization. Duquesne K, Sturgis JN. Methods
Biochim Biophys Acta. 1975; 415(1):29-79. Mol Biol. 2010;601:205-17.
25. Strategies for the purification of membrane proteins. Smith SM. Me-
4. The fluid mosaic model of the structure of cell membranes. Singer thods Mol Biol. 2011; 681:485-96.
SJ, Nicolson GL. Science. 1972; 175(4023):720-31. 26. Detergent solubilization of lipid bilayers: a balance of driving forces.
Lichtenberg D, Ahyayauch H, Alonso A, Goñi FM. Trends Biochem
5. The basic structure and dynamics of cell membranes: an update of Sci. 2013;38(2):85-93.
the Singer- Nicolson model. Goñi FM. Biochim Biophys Acta. 2014; 27. The mechanism of detergent solubilization of lipid bilayers. Lich-
1838(6):1467-76. tenberg D, Ahyayauch H, Goñi FM. Biophys J. 2013; 105(2):289-
99.
6. On bimolecular layers of lipoids on the chromocytes of the blood. 28. Physical principles of membrane organization. Israelachvili JN, Mar-
Gorter E, Grendel F. J Exp Med. 1925; 41(4):439-43. celja S, Horn RG. Q Rev Biophys. 1980; 13(2):121-200.
29. Theory of self-assembly of hydrocarbon amphiphiles into micelles
7. Surface tension. Pokels A. Nature 1891; 46:437-39. and bilayers. Israelachvili, JN, Mitchell DJ, Ninham DW. J. Chem.
8. Reconstitution of cell membrane structure in vitro and its transfor- Soc. Faraday Trans. II 1976; 72,1525–1568.
30. Surfactant-induced release of liposomal contents. A survey of me-
mation into an excitable system. Mueller P, Rudin DO, Tien HT, Wes-
cott WC. Nature. 1962; 194:979-80.
9. Supported phospholipid bilayers. Tamm LK, McConnell HM. Biophys
J. 1985; 47(1):105-13.
10. Atomic force microscopy of supported planar membrane bilayers.
Singh S, Keller DJ. Biophys J. 1991; 60(6):1401-10.
11. Diffusion of univalent ions across the lamellae of swollen phospho-
lipids. Bangham AD, Standish MM, Watkins JC. J Mol Biol. 1965;
13(1):238-52.
12. Studies on membrane fusion. I. Interactions of pure phospholipid
membranes and the effect of myristic acid, lysolecithin, proteins and
dimethylsulfoxide. Papahadjopoulos D, Hui S, Vail WJ, Poste G. Bio-
chim Biophys Acta. 1976;448(2):254-64.
13. Production of large unilamellar vesicles by a rapid extrusion proce-
Detergentes: de los principios físicos a las aplicaciones 91
biofarmacéuticas (o por qué prevenimos la covid-19 con agua y jabón)
Félix M. Goñi y Alicia Alonso
An. Real Acad. Farm. Vol. 87. Nº1 (2021) · pp. 53 - 96
