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261ANALESRANFwwwanalesranfcomMaternalfructoseintakemodulatescholesterolmetabolisminresponsetoaWesterndietintheoffspringPalomaLópezLaizElenaFausteetalAnRealAcadFarmVol88nº3(2022)·pp257-275groupreceiveda10%fructosesolution(w/v)whilethecontrolgroup wasprovided with tap water. Thenafterparturition thelitterwas standardised tonineratsandboth themotherand theoffspringdrankwater.Thelactationfinishedatday21andonlythemaleratswerekeptuntilthreemonthsofageTheyreceivedastandarddietand water withoutany supplementOnce theyreached 3monthsofagemaleratswerealsoseparatedintovariousgroupsdependingonthediet–Controlgrouptheyconsumedwaterwithoutadditivesandastandarddiet–Fructosegroup theyreceiveda10%fructosesolution(w/v)andastandarddiet–Tagatosegrouptheyconsumeda10%tagatosesolution(w/v)andastandarddiet–Fructosepluscholesterolgroup they weregivena10% fructose solution(w/v)anda standarddiet supplemented with 2%cholesterolIn totalsince thematernal treatmentwasalso takenintoaccounttherewere8experimentalgroupsofmaleratswhichareshowninFigure 4 After the 21daysofdiet therats were sacrificedTheirbloodwascollectedinEDTA-K2tubeswhichwascentrifugedtoseparatetheplasmaAlsotheirliverswereweightedBothplasmaandliverswerefrozenat–800Cfortheirlateruse32Biochemicalparameterassays32aTotalplasmacholesteroldeterminationIt wasperformed withanenzymaticcolorimetricend-pointassay,usingaSpinreact(Spain)kitSomestudieshavealreadyshownthedeleteriouseffectthatfructoseconsumptionduringgestationcanproduceintheprogenydyslipidaemia(43)lipidsoxidative stress(44)modifiedleptinsignalling(45)andimpairedhippocampalfunction(46)Moreover,theoutcomecanbegender-dependentasitisshowninastudy(47)thatdemonstratedthatcholesterollevelsvarieddependingonthesexoftheanimal2OBJECTIVESTheobjectivesofthepresentworkare– To study whether fructoseintakebypregnantratsduringgestationhasanyeffectoncholesterolmetabolismofthemaleoffspring– Todetermine whether fructose(or tagatose)and fructosepluscholesterolintakebymaledescendantratsproducesanyvariationin theircholesterolmetabolismandin thiscase to studywhetherthesechangesareaffectedbythematernaldiet3MATERIALSANDMETHODS31ExperimentaldesignTheexperimentwasapprovedbytheEthicsCommitteeTheexperimentaldesignwascarriedoutasfollowsFirst femaleandmale Sprague-Dawleyrats werematedOncegestation wasconfirmed femalepregnantrats wereprovidedwithastandarddietand theywereseparatedin twogroupsaccordingtothedrinktheyreceivedthroughoutgestationThefructoseFigure4ExperimentalDesignCreatedwithBioRender.com