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P. MADRIGAL Y COLS. AN. R. ACAD. NAC. FARM.

Isocratic HPLC conditions used along this experiment were: the

mobile phase consisted of a 20:80% solution (v/v) of methanol in

0.01 M PO4H2K. The pH of the final solution was adjusted to 3.2 with
phosphoric acid. The flow rate was 1 mL/min. Chromatograph was

loaded with 40 µL of each sample. Chromatograms were run at room
temperature. Samples were detected at 254 nm.

RESULTS AND DISCUSSION

Concentration of cefotaxime

The concentration homogenates of free cefotaxime was measured
in blood, liver, kidney, spleen, lung and heart of the killed rats at
the 30, 60, 90, 120, 150, 180 and 210 min. after the administration
of cefotaxime, those rats were the rats with vanadium and antibiotic
and the rats with cefotaxime only. As there were 5 rats by each time,
the average concentration of free cefotaxime was calculated by each
group of 5 rats that were killed at each time.

TABLE I. Average concentrations (mg/mL) of free cefotaxime in rats without
vanadium

Time (Min)

Samples 30 60 90 120 150 180 210

BLOOD 0.80 0.46 0.25 0.14 0.08 0.06 0.02

LIVER 6.03.10–5 2.68.10–5 2.16.10–5 1.71.10–5 1.42.10–5 1.37.10–5 1.24.10–5

SPLEEN 2.70.10–5 1.58.10–5 1.45.10–5 0.53.10–5 0.41.10–5 0.39.10–5 0.38.10–5

KIDNEY 24.2.10–5 18.1.10–5 10.6.10–5 5.90.10–5 5.01.10–5 4.81.10–5 3.17.10–5

LUNG 7.19.10–5 5.26.10–5 3.52.10–5 3.02.10–5 1.33.10–5 1.22.10–5 0.46.10–5

HEART 2.56.10–5 1.87.10–5 1.23.10–5 1.14.10–5 1.08.10–5 0.86.10–5 0.85.10–5

In blood and in the rest of organs such as liver, spleen, lung,
heart and kidney, we can observe in the Table I that the cefotaxime

604