Page 60 - 72_04

P. MADRIGAL Y COLS. AN. R. ACAD. NAC. FARM.

MATERIALS AND METHODS

Biology Materials

Seventy five Wistar rats, males of 250 to 300g weight, were used
throughout this work and supplied by the warehouse of the
Complutense University of Madrid.

Animals were maintained in one of the rats room of the
warehouse. The temperature was 20º C, the relative humidity was
55% and the intensity of the light inside the room was 400 LUX
using cycles of 12 hours of light/darkness.

Animals were distributed by groups in order to assay different
treatments. One group of 35 rats with vanadium (group A), which
was poisoned with a nasogastric sound administering to their
everyday 4 mL of a solution of 4 mg/mL of a vanadium (III)
acetylacetonate during 8 days. The groups left were arranged one of
them with 35 rats (group B), and the other one with 5 rats (group C),
both of them without metal.

Groups A and B were put under treatment with an intramuscular
administration of an only dose of 200 mg of cefotaxime.

Group C was used as a control to know the different substances
which belonged to the animals in order to not to be confusing with
the free cefotaxime throughout the study.

Five rats of the groups A and B, were sacrificed after 30, 60, 90,
120, 150, 180 and 210 minutes after the antibiotic administration.
The blood, liver, spleen, kidney, lung and heart of those rats were
extracted and after that, they were measuring their free cefotaxime
concentration by HPLC.

Chemical and Reagents

Cefotaxime sodium was supplied by the Teaching Hospital of
Madrid. Vanadium (III) acetylacetonate was obtained from Merck.
Trichloracetic acid supplied by Scharlau, this substance was used in
concentrations of 10% and 20%.

602