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VOL. 72 (4), 599-609, 2006 VANADIUM IN VIVO INTERACTION WITH CEFATOXINE

FIGURE 1. Mechanism of hydrolytic reaction of the Cefotaxime with addition of
vanadium.

So that we could reach those results, we compared the
concentration, distribution and stability of the free cefotaxime in
rats without toxic with poisoned rats, after 30, 60, 90, 120, 150, 180
and 210 min. after administrating this antibiotic. This comparison
was achieved by measuring the concentration of free cefotaxime
in blood, liver, spleen, kidney, lung and heart homogenates of
poisoned rats and rats without the toxic. The methodology applied
for this purpose was HPLC since this technique facilitates the
separation of the complex V(IV)-cefotaxime, the corresponding
V(IV)-cephalosporanic acid chelate, free cefotaxime and their
compounds resulting from the degradation of cefotaxime.

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