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V. ESTEPA; S. RÓDENAS; M.C. MARTÍN ANAL. REAL ACAD. FARM.
introduciéndose modificaciones que afectan principalmente al tiempo de reacción y al
método de extracción de los lipoperóxidos.
Palabras clave: Aductos MDA-TBA.- Peroxidación lipídica.- Reacción del TBA.
SUMMARY
A optimized method for the analysis of the lipidic peroxidation in human serum
A variety of free radicals are produced in human organism as a consequence of
normal cell metabolism. These free radicals are generally removed by endogenous
scavengers but also can interact with plasma and tisular lipids causing its peroxidation.
It is very difficult to determine the magnitude of the peroxidation and also the
first lipidic peroxidation products due to their very short half-life. However the
evaluation of the secondary lipidic peroxidation products can be quantitatively
determined as well as their degradation products such as aldehydes with high reactivity
among them malondialdehyde (MDA) is the most relevant.
Among the variety of assays developed to estimate the magnitude of oxidative
damage in humans, the most commonly employed is based on the reaction of
endogenous malondialdehyde (MDA) with 2-thiobarbituric acid (TBA). This reaction
yields very stable cromogenic and fluorogenic MDA-TBA adducts, that are detectable
by UV spectrophotometry and fluorimetry.
In the present study, a colorimetric method described by ASAKAWA and
MATSUSHITA, was optimized to determine the human serum lipoperoxides based on
the TBA reaction. The reaction conditions established by these authors were rigorously
investigated in order to optimize this procedure. Mainly, changes were introduced
relating to the reaction time and the lipoperoxides extraction method.
Key words: MDA-TBA aducts.- Lipidic peroxidation.- TBA reaction.
INTRODUCCIÓN
En el organismo humano, como consecuencia del metabolismo
celular normal, se producen radicales libres que generalmente son
eliminados por receptores endógenos pero también pueden interaccionar
con los lípidos séricos y tisulares provocando su peroxidación. La
peroxidación lipídica juega un papel importante en la patogénesis y
gravedad de diversas enfermedades.
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