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ANALESRANFwww.analesranf.comwith only the ethanol extract (3).5. CONCLUSIONS In the present research, important metaboliteswere identified in the anti-inflammatory activitysuch as flavonoids, tannins, saponins, andcoumarins, which are crucial for the antiinflammatory property and are of greatimportance for the evaluation of various importantpharmacological activities for the treatment ofother pathologies, since there are no studies thatdetermine such activity with the ethanolic extractof baccharis macrantha, making it innovative andinteresting to conduct more research on thetreated species. Gels based on the leaves of theethanolic extract of baccharis macrantha showedanti-inflammatory activity when evaluated inmice, using the plantar edema method induced by0.5% carrageenan. Regarding the three gelsformulated, it was demonstrated that the gelformulation at 18.75% has a greater antiinflammatory effect, confirmed by the ANOVAstatistical analysis and the Tukey Test, which alsocomplied with all the physicochemical andmicrobiological quality controls established by theUSP. Therefore, by complying with all theparameters and proving its anti-inflammatoryactivity, the gels could be used as a treatment inhumans with acute and possibly severeinflammatory processes, providing quick andRegarding the physicochemical characteristicsof the gels, they had an acidic pH within theestablished range (10), which states that gelsshould be within a pH range of 4-7. Regardingviscosity, there is no specific value, as they havetheir own characteristics. Regarding extensibility,indicated that the value should be a maximum of5 cm for the average radius, and all concentrationswere within normal values (11). Formicrobiological control is there should be amaximum limit of 10,000 CFU/g for mesophilicaerobes and >100 for fungi and yeasts, and the gelsproduced were within the required range (12). Theabsence of total coliforms, mesophilic aerobes,fungi, and yeasts in the Baccharis latifolia gel (13).In the study of Baccharis, the highestconcentration formulation (7 mg/g or 7%) showeda greater anti-inflammatory effect, and statisticalanalysis using ANOVA indicated a significantdifference, with the 7% gel showing greater antiinflammatory activity than the other treatments.It had the same anti-inflammatory power as itscontrol group. Therefore, both species, Baccharislatifolia and baccharis macrantha, have antiinflammatory activity, with differentconcentrations, but still possessing antiinflammatory properties. Also evaluated theanti-inflammatory activity of the same species,but using the red blood cell stabilization methodEvaluation of the anti-inflammatory activity of gelsGabriela F. Castelo, Gisella A. Paredes et al. 337 An. R. Acad. Farm.Vol. 90. n%u00ba 3 (2024) %u00b7 pp. 329-338Figure 6. Simultaneous Tukey confidence intervals of inflammation percentages of treatment groups in anti-inflammatory evaluation