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OTERO, Y. F. & DE LAS HERAS, B. AN. R. ACAD. NAC. FARM.

na por ácidos grasos en cardiomiocitos podría contribuir a la intol-
erancia a la glucosa asociada a los estados de obesidad que cursan
con diabetes tipo 2.

Palabras clave: Cardiomiocitos; Ácidos grasos; AMPK; ACC; Glucosa.

ABSTRACT

Insulin signaling study in new cellular model from myocardium

In the last years, the prevalence of obesity and metabolic diseases
like diabetes type 2 and metabolic syndrome, suffered an increase.
Underlying effect in people with obesity with or without diabetes type
2, are insulin resistance and cardiovascular disease that are one of the
major cause of death. Nevertheless, the action of insulin resistance
over cardiac function is unknown. In this work, we studied the insulin
signaling and its resistance in mice neonatal cardiomyocytes. We
generated a new cellular line of immortalized cardiomyocytes. In these
cells we studied the expression of several proteins of insulin signaling
pathway like insulin receptor and their major substrates IRS-1 and
IRS-2, besides PKB/Akt pathway, ERKs and p70/S6K. We also studied
some ß-oxidation enzymes like Acetyl-CoA Carboxilase and AMPK.
Finally, we studied glucose uptake with 2-Deoxy-glucose under
differents stimuli. The results showed that our new cell line are insulin
and free fatty acids sensitive but it presents insulin resistance to
glucose uptake which can be due to a low expression of GLUT4. In
conclusion, these new cellular model may be useful to study glucose
intolerance associated to obesity that appears in diabetes type 2.

Key Words: Cardiomyocytes; Fatty acids; AMPK; ACC; Glucose.

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