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VOL 66 (4) 2000 IN VITRO INDUCTION OF APOPTOSIS BY CYCLOSPORINE A

Fig. 3: Effect of CsA on chromatin fragmentation in hepatocytes after 4 hours (open
square) and 20 hours (closed square). Data are expressed as mean r SD (n=3).
Statistically significant differences versus the control group are expressed as
*P0.05, **P0.01 and ***P0.001.

DNA fragmentation, as determined by the TUNEL assay, showed in a
time- and dose-dependent increase after treatment with CsA. At both
time points, 50 µM CsA caused a percentage of positive TUNEL

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