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ARMIN WOLF ANAL REAL ACAD. FARM.

Fig. 2: Effect of CsA on chromatin condensation in hepatocytes after 4 hours
(open square) and 20 hours (closed square). Data are expressed as mean r SD
(n=3). Statistically significant differences versus the control group are

expressed as *P0.05 and ***P0.001.

Chromatin fragmentation in control hepatocytes was 0.1 % after 4
hours and 0.6 % after 20 hours. Under the same conditions, CsA
caused a dose-dependent increase in fragmented nuclei. After 20
hours of incubation, 50 µM CsA increased chromatin fragmentation,
compared with controls by a factor of 7.4. The effects observed at all
concentrations after 20 hours were, on average, about 2-3 times higher
than after 4 hours (Fig.3)

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