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VOL 66 (4) 2000 IN VITRO INDUCTION OF APOPTOSIS BY CYCLOSPORINE A

Fig. 1: Effect of CsA on release of lactate dehydrogenase after 4 hours (open square) and
20 hours (closed square). Data are expressed as mean r SD (n=3). Statistically
significant differences versus the control group are expressed as ***P0.001

Morphology
After 4 and 20 hours of cultivation, untreated control hepatocytes

showed a percentage of 1-2 % condensed chromatin. Incubation of the
hepatocytes with CsA resulted in a dose-dependent increase in chromatin
condensation, with maximum percentages reached at concentrations of 50
µM, i.e. 8.5 times and 14 times above control values after 4 hours and 20
hours, respectively (Fig. 2).

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