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Céspedes observed inhibitions with good results from di- ANALES
luting 400 µg of compound. However, I did not achieve total inhi- RANF
bitions at said study concentration. On the other hand, with the
Shigella bacteria they failed to obtain growth inhibition. In our study, www.analesranf.com
carried out from 75 µl of macerate, a partial inhibition of Shigella
was achieved, something that was not observed in the Céspedes 3. Manandhar S., Luitel S., Kumar-Dahal R., In vitro antimicrobial ac-
study. Thus, the difference in the secondary metabolites, as well as tivity of some medicinal plants against human pathogenic bacteria.
the mixture of the same in the macerate, have potentiated the effect, J. Trop. Med. 2019: 1895340.
and thus achieved an inhibition of Shigella, and a total inhibition https://doi.org/10.1155/2019/1895340
of both enterobacteria from 100 µl of extract.
4. Cicció J. F. A source of almost pure methyl chavicol:volatile oil from
Another point that could reflect the lack of activity on the the aerial parts of Tagetes lucida (Asteraceae) cultivated in Costa
part of the molecules studied by Céspedes is that both strains of Shi- Rica Rev. Biol. Trop, 2004: 52(4), 853-857.
gella are wild-type strains, and the resistance to certain compounds
can vary not only from one geographical area to another, but also 5. Pérez-Ortega, G., González-Trujano, M. E., Ángeles-López, G. E., et
between the type of sample of isolated, and the genotypes and phe- al. Tagetes lucida Cav.: Ethnobotany, phytochemistry and pharma-
notypes expressed. cology of its tranquilizing properties. Journal of Ethnopharmacology,
5. CONCLUSION 2016: 181: 221–228. doi:10.1016/j.jep.2016.01.040
The use of extracts of medicinal plants that have antimi- 6. Kurpis, J., Serrato Cruz, M. A., Feria Arroyo, T. P. Modeling the ef-
crobial activity suggests an alternative under study to combat infec- fects of climate change on the distribution of Tagetes lucida Cav. (As-
tious diseases resistant to antibiotics. teraceae). Global Ecology and Conservation, 2019: e00747.
doi:10.1016/j.gecco.2019.e00747
It suggests a phytochemical panel for determining the ac-
tive metabolites of the extract and evaluating which is the most active 7. Céspedes, C. L., Avila, J. G., Martínez, A., Serrato, B., et al. Antifun-
against bacterial growth. As well as elucidating the mechanism by gal and antibacterial activities of mexican tarragon (Tagetes lucida).
which such inhibition is achieved and whether it also inhibits the re- Journal of Agricultural and Food Chemistry, 2006: 54(10): 3521–
lease of virulence factors. It was found that the solvent did not affect 3527. doi:10.1021/jf053071w
growth, different concentrations of the macerate were evaluated.
6. FINANCING 8. Jennison, A.V. Verma, N. K. Shigella flexneri infection: pathogenesis
and vaccine development, FEMS Microbiology Reviews, 2004:
The following research work was not funded by any insti- 28(1): 43–58. doi.org/10.1016/j.femsre.2003.07.002
tution.
7. ACKNOWLEDGMENT 9. Nisa, I., Qasim, M., Yasin, N., et al., Shigella flexneri: an emerging
pathogen. Folia Microbiologica. 2020, 65, 275–291.
We thank all authors and Universities immensely for their doi:10.1007/s12223-020-00773-w
support in carrying out this project. And We dedicate this work es-
pecially to Victoria daughter of the main authors. 10. Qi Jin., et al., Genome sequence of Shigella flexneri 2a: insights into
pathogenicity through comparison with genomes of Escherichia coli
K12 and O157., Nucleic Acids Research, 2002: 30(20): 443-4441
doi.org/10.1093/nar/gkf566
11. Xiao-Ying Pu., et al., Characterization of fluoroquinolone-resistant
Shigella flexneri in Hangzhou area of China. Journal of Antimicrobial
Chemotherapy, 2009: 63: 917–920 doi:10.1093/jac/dkp087
12. Min-Soo K., et al., A study on the prevention of salmonella infection
by using the aggregation characteristics of lactic acid bacteria., Toxi-
col Res., 2013: 29(2): 129–135. doi: 10.5487/TR.2013.29.2.129
13. Everest, P., Wain, J., Roberts, M., et al. The molecular mechanisms
of severe typhoid fever. Trends in Microbiology, 2001: 9(7): 316–
320. doi:10.1016/s0966-842x(01)02067-4.
CONFLICT OF INTERESTS
8. REFERENCES The authors deny having problems due to a conflict of interest.
1. Arroyo Mejía M. F., Valdez Arcos O., Almeida Villegas J.A., Resis- Si desea citar nuestro artículo:
tance to antibiotics; a perspective, Rev. Med. Inv., 2019: 7(2): 67- Jorge Angel Almeida-Villegas; Rodolfo Garcia-Contreras; et al.
72 Effect of the hexanic extract of Tagetes lucida on the
inhibition of the growth of enterobacteria Shigella
2. Arroyo Mejía M. F., López López O. O., Almeida Villegas J.A., Resis- flexneri and Salmonella typhiAn Real Acad Farm [In-
tencia a antibióticos. Breve revisión. MedLab., 2019: 11 (4): 3-12 ternet].
Effect of the hexanic extract of Tagetes lucida on the inhibition An Real Acad Farm Vol. 86. Nº 2 (2020) · pp. 113 -115
of the growth of enterobacteria Shigella flexneri and Salmonella
typhi DOI: http://
Jorge Angel Almeida-Villegas; Rodolfo Garcia-Contreras; et al.
116 An Real Acad Farm Vol. 86. Nº 2 (2020) · pp. 113 -116
