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ANALESRANFwww.analesranf.com489 An. R. Acad. Farm.Vol. 90. n%u00ba 4 (2024) %u00b7 pp. 489-498ART%u00cdCULO DE INVESTIGACI%u00d3NIdentification of Staphylococcus aureus and prevalence ofStaphylococcus aureus resistant to methicillin and other patterns ofresistance to antibiotics in clinical isolates of diabetic footIdentificaci%u00f3n de Staphylococcus aureus y prevalencia de Staphylococcusaureus resistente a meticilina y otros patrones de resistencia a antibi%u00f3ticosen aislamientos cl%u00ednicos de pie diab%u00e9ticoEdder Guadalupe Segura Ram%u00f3n1, Gabriel Mart%u00ednez Gonz%u00e1lez2, Jorge Almeida31Bacherlor in Q.F.B. University of Ixtlahuaca CUI. Ixtlahuaca Jiquipilco, San Pedro, 50740 Ixtlahuaca de Ray%u00f3n, Mexico. 2Professor-Researcher of the University of Ixtlahuaca CUI-Bachelor of Science in QFB-INIES. Ixtlahuaca Jiquipilco, San Pedro, 50740 Ixtlahuaca de Ray%u00f3n, Mexico. 3Professor of the University of Ixtlahuaca CUI-Bachelor ofMedicine, Nutrition and Q.F.B. Ixtlahuaca Jiquipilco, San Pedro, 50740 Ixtlahuaca de Ray%u00f3n, Mexico.e-mail: angelalmeidavillegas@gmail.comRecibido el 27 de diciembre de 2024; aceptado el 10 de enero de 2025Disponible en internet el 31 de enero de 2025ABSTRACTObjective: To determine the prevalence of methicillin-resistant Staphylococcusaureus (MRSA) in diabetic foot wounds and analyze antibiotic resistance patterns.Introduction: The global incidence of diabetes mellitus, particularly type II, hassignificantly increased, leading to complications such as diabetic foot ulcers.These ulcers often become infected, with MRSA being a common and challengingpathogen. Understanding the prevalence and resistance patterns of MRSA indiabetic foot infections is crucial for effective treatment.Methodology: Diabetic foot wound samples were collected from 65 patients inthe Valley of Toluca, Mexico. Samples were cultured and analyzed usingchromogenic agar, BHI, salt and mannitol, calf blood, EMB, and MacConkey agars.Strain identification and antibiotic sensitivity testing were performed using theVytek automated system. Oxacillin and cefoxitin were used to detect methicillinresistance. Statistical analyses, including Kolmogorov-Smirnov, Shapiro-Wilktests, and Spearman correlation, were conducted to evaluate relationshipsbetween clinical factors and antibiotic resistance.Results: Of the 65 samples, 56.9% were from men and 43.1% from women, with97.1% having type II diabetes. MRSA prevalence was 63%. Resistance rates werehighest for ampicillin (100%), clindamycin (89%), erythromycin (87%), andgentamicin (73%). Statistical analysis showed no significant correlation betweenoxacillin resistance and glycemic control, erythromycin resistance (MLSresistance), hypertension, or gender.Conclusion: The high prevalence of MRSA in diabetic foot wounds underscoresthe need for targeted infection control and appropriate antibiotic therapy. Thelack of correlation between resistance and clinical factors suggests amultifactorial nature of antibiotic resistance, necessitating broader clinical andmicrobiological considerations for future studies.DOI:https://doi.org/10.53519/analesranf.ISSN: 1697-4271 E-ISSN: 1697-428X/Derechos Reservados %u00a9 2024 Real Academia Nacional de Farmacia. Este es un art%u00edculo de acceso abierto A N A L E S D E L AR E A L A C A D E M I AN A C I O N A L D E F A R M A C I AKEYWORDS Diabetic FootBacterial resistanceMRSA