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492ANALESRANFwww.analesranf.comIdentificaci%u00f3n de Staphylococcus aureusy prevalencia de Staphylococcus aureus resistente a meticilina Jorge Almeida et al.An. R. Acad. Farm.Vol. 90. n%u00ba 4 (2024) %u00b7 pp. 489-498explanation of why, are criteria and factorsthat are not contemplated for the presentstudy.The primary swab was replated onchromogenic agar, BHI, salt and mannitol, calfblood, EMB and MacConkey, 6 hours after thesample was taken. In addition to the microbialreplating, a Gram stain was performed oneach of the samples. The Petri dishes wereplaced in an incubation oven for 18 hours at37%u00b0C.Once the replatings were obtained, withinapproximately 18 hours, the strain wasidentified, as well as the sensitivity toantibiotics with an automated method usingthe VITEK%u00ae 2 equipment. For the tests oftested antibiotics and concentrations, the CLSIm100 standard was followed.To detectresistance to methicillin, the oxacillin 1 %u00b5gtest and the cefoxitine 30 %u00b5g test were used.For the rest of the antibiotics, the followingwere tested at the indicated concentrations(penicillin 10 %u00b5g, ampicillin 10 %u00b5g,ampicillin/sulbactam 8/4%u00b5g,amoxicillin/clavulanic 10 %u00b5g, ceftriaxone30%u00b5g, oxacillin 1 %u00b5g, cefoxitin 30 %u00b5g,clindamycin %u00b5g, erythromycin 15 %u00b5g,gentamicin 10 %u00b5g, tetracycline 30 %u00b5g,levofloxacin 5 %u00b5g, ciprofloxacin 5 %u00b5g,mofloxacin 5 %u00b5g, rifampin 1 %u00b5g,trimethoprim/sulfamethoxazole 1.25/23.75%u00b5g, linezolid 4 %u00b5g, daptomycin 1 %u00b5g).In this study, the relationship betweenoxacillin resistance and several clinical factorswas analyzed, including glycemic control,erythromycin resistance, arterial hypertension(HTN) and the gender of the patients. Todetermine the normality of the data, theKolmogorov-Smirnov and Shapiro-Wilk testswere used and the Spearman correlationcoefficient was applied to evaluate thepossible relationships between the variables.Statistical analyzes were performed using IBMSPSS Statistics for Windows, version 21.0 (IBMCorp., Armonk, NY).3. RESULTSOf the 100% of the sample (n=65), 56.9%(n=37) were men and 43.1% (n=28) werewomen, of which 97.1% (n=63) had type 2diabetes mellitus and only 2.9% (n=2) had type1 diabetes mellitus. Among concomitantdiseases, 27.7% (n=18) had additional arterialhypertension, all of them in 100% of peoplewith type 2 diabetes mellitus. Forcardiovascular disease, only 16.9% (n=11) werepatients with type 2 diabetes. Glycemiccontrol of patients is not something thatdepends on this study, that follow-up orprescription has been given, however,according to the questionnaire, it was thefollowing: for people with type 1 diabetesmellitus, 100% had control using insulin plusan oral hypoglycemic, prescribed as such bytheir doctor. treating. For patients with type2 diabetes mellitus, only 40% (n=26)maintained control. Of the sample, there arepatients with controlled DM 2, only 10.7%(n=7) use insulin for glycemic control and29.3% (n=19) have glycemic control withunspecified oral hypoglycemic agents. Of theentire sample, only 10.7% (n=7) have had morethan one reinfection, 1.5% (n=1) of the peoplewho did not present recurrence had MRSA.Table 1 shows the characteristics of thepatients in the study. For the antibiotics tested, 100% (n=65)resistance to ampicillin was obtained, 86%(n=56) for ampicillin/sulbactam, which, asdescribed below, suggests the presence ofbeta-actamases. In order of resistance,clindamycin showed 89% (n=58) resistance anderythromycin 87% (n=57) suggestive of MLStype resistance. For the aminoglycosidegentamicin it was 73% (n=48), tetracyclineshowed 67% (n=44), in the case of betalactams and MRSA markers; penicillin,amoxicillin/clavulanic acid, ceftriaxone andoxacillin, as well as the cefoxitin test, thereis 63% (n=41) resistance. The quinoloneslevofloxacin and ciprofloxacin showed 56.9%(n=37) and mofloxacin 50.7% (n=33), rifampin