Page 123 - 80_02
P. 123
The
role
of
the
catecholaminergic…
Figure
8.
Effect
of
TH
on
anterior--posterior
heart
tube
patterning
and
specification
of
chambers.
Effect
of
TH
gain--of--function
on
sino--atrial
gene
expression.
Embryos
from
stages
10
or
12
underwent
in
situ
hybridization
for
the
indicated
gene
markers,
AMHC1
and
Tbx5.
The
control
non--electroporated
embryos
are
compared
with
embryos
electroporated
with
either
the
control
construct
(pCAGs--I--GFP)
or
with
the
th
expressing
construct
(pCAGs--TH--I--GFP).
The
anterior
expanded
expression
of
AMHC1
and
Tbx5
in
the
heart
tube
is
indicated
by
the
black
arrows.
Visualization
of
GFP
expression
for
the
embryos
electroporated
is
shown
in
the
corresponding
left
panels.
(Modified
from
[13]).
It
is
worth
mentioning
that
the
th
global
knockout
mice,
generated
in
1995
by
the
group
of
Dr.
Palmiter
(21),
is
embryonic
lethal
starting
at
day
E11.5--12.5.
Although,
the
null
mice
die
from
apparent
heart
failure,
a
detailed
characterization
of
the
mouse
embryo
phenotype
in
organogenesis
has
not
been
carried
out
yet.
This
type
of
study
would
be
required
to
unravel
the
role
of
catecholamines
in
mouse
cardiogenesis.
359
