Page 119 - 80_02

The
role
of
the
catecholaminergic…

Figure
6.
Expression
of
receptors
in
the
chick
embryonic
heart
regions.
A)
Analysis
of

adrenergic
receptors
(a-­-1b,
a-­-1d
and
a-­-2c
;
ß-­-1
and
ß-­-2)
expression
by
RT-­-PCR
of
mRNA
from
the

precardiogenic
area
(stage
5),
endocardial
tubes
(stage
8)
or
heart
tube
(stage
10).
An
internal

control
with
GAPDH
is
shown
below.
B)
Analysis
of
dopaminergic
receptors
(D1,
D3
and
D5)

expression
by
quantitative
PCR
of
mRNA
from
stage
10
and
12
heart
tubes.
The
levels
of
dopamine

receptor
transcripts
were
normalized
relative
to
GAPDH
mRNA
levels.
Primers
are
shown
in
Tables

1
and
2.

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