Page 118 - 80_02

C.
Hernández-­-Sánchez,
P.
Vázquez,
Flora
de
Pablo

TH
as
well
as
adrenergic
and
dopaminergic
receptors
are
expressed
in
the
chick

embryo
developing
heart.

We
have
found
that
th
mRNA
expression
is
enriched
in
the
cardiac
field
of

gastrulating
chick
embryos.
By
stages
8-­-10,
it
was
restricted
to
the
splanchnic

mesoderm
of
both
endocardial
tubes
and
was
subsequently
(stages
11-­-12)

expressed
predominantly
in
the
myocardial
layer
of
the
atrial
segment
(Figure
5,

(13)).
The
levels
of
th
mRNA
in
the
heart
increased
several
fold
between
stage
8

and
stage
12
(Figure
5).

Figure
5.
Tyrosine
hydroxylase
expression
in
the
chick
embryonic
heart.
In
the
upper
panel,

whole
mount
in
situ
hybridization
for
stages
10
to
12
are
shown.
Note
the
restricted
expression
in

the
heart
tube.
In
the
lower
panel
RT-­-PCR
of
th
mRNA
expression
is
shown
in
the
developing

endocardial
tube
(stage
8)
and
heart
tube
(stages
10
and
12)
and
postnatal
day
10
(P10)
heart.
An

internal
control
with
GAPDH
is
shown
below.
(Modified
from
[13]).

354