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C.
Hernández-­-Sánchez,
P.
Vázquez,
Flora
de
Pablo

B

Figure
2.
Novel
forms
of
gene
regulation
in
the
th-­-ins
locus.
A)
The
schematic
genomic

organization
of
the
chicken
th-­-ins
locus
(there
is
a
similar
cluster
in
mammals
although
the

transcriptional
regulation
may
be
different)
and
diagram
of
the
transcripts
generated
by
the
out-­-of-­-
frame
splicing
between
th
exon
13
and
exon
2
or
3
of
the
insulin
gene.
B)
TH
isoforms
generated

and
their
enzymatic
activity
was
analyzed
by
HPLC
for
L-­-DOPA
production.
The
extracts
of

transfected
HEK293T
cells
with
these
TH
isoforms
show
a
lower
enzymatic
activity
than
the

canonical
TH.
(Modified
from
[8])

350