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  Reduction	
  in	
  mitochondrial	
  membrane	
  peroxidizability	
  index…	
  

	
  

          Patridge,	
   L.;	
   Gems,	
   D.;	
   Wither,	
   D.J.	
   Ribosomal	
   protein	
   S6	
   kinase	
   1	
   signaling	
   	
   	
   	
   regulates	
  
          mammalian	
  life	
  span.	
  Science	
  2009;	
  326,	
  140–144	
  

     21.	
   	
   	
   	
   Okumura,	
   S.;	
   Suzuki,	
   S.;	
   Ishikawa,	
   Y.	
   New	
   aspects	
   for	
   the	
   treatment	
   of	
   Cardiac	
   Diseases	
  
          based	
   on	
   the	
   diversity	
   of	
   functional	
   controls	
   on	
   cardiac	
   muscles:	
   Effects	
   of	
   targeted	
  
          disruption	
  of	
  the	
  type	
  5	
  adenylyl	
  cyclase	
  gene.	
  J	
  Pharmacol	
  Sci	
  2009;	
  109,	
  354-­-359	
  

     22.	
   	
   	
   	
   Porter,	
   A.G.;	
   Urbano,	
   A.G.	
   Does	
   apoptosis-­-inducing	
   factor	
   (AIF)	
   have	
   both	
   life	
   and	
   death	
  
          functions	
  in	
  cells?	
  Bioessays	
  2006;	
  28,	
  834-­-843	
  

     23.	
  	
  	
  	
  Barja,	
  G.	
  Free	
  radicals	
  and	
  aging.	
  Trends	
  in	
  Neurosci	
  2004;	
  27,	
  595-­-600	
  

     24.	
   	
   	
   	
   Gredilla,	
   R.;	
   Sanz,	
   A.;	
   Lopez-­-Torres,	
   M.;	
   Barja,	
   G.	
   Caloric	
   restriction	
   decreases	
  
          mitochondrial	
   free	
   radical	
   generation	
   at	
   Complex	
   I	
   and	
   lowers	
   oxidative	
   damage	
   to	
  
          mitochondrial	
  DNA	
  in	
  the	
  rat	
  heart.	
  FASEB	
  J	
  2001;	
  15,	
  1589-­-1591	
  

     25.	
   Barja	
   G.;	
   Herrero	
   A.	
   Oxidative	
   damage	
   to	
   mitochondrial	
   DNA	
   is	
   inversely	
   related	
   to	
  
          maximum	
  life	
  span	
  in	
  the	
  heart	
  and	
  brain	
  of	
  mammals,	
  FASEB	
  J,	
  2000;	
  14,	
  312-­-318	
  

     26.	
   Ruiz,	
   M.C.;	
   Ayala,	
   V.;	
   Portero-­-Otín,	
   M.;	
   Requena,	
   J.R.;	
   Barja,	
   G.;	
   Pamplona,	
   R.	
   Protein	
  
          methionine	
  content	
  and	
  MDA-­-lysine	
  adducts	
  are	
  inversely	
  related	
  to	
  maximum	
  life	
  span	
  in	
  
          the	
  heart	
  of	
  mammals.	
  Mech	
  Ageing	
  Dev	
  2005;	
  126,	
  1106-­-1114	
  

     27.	
   	
   	
   	
   Bell,	
   E.L.;	
   Guarente,	
   L.;	
   The	
   SirT3	
   divining	
   rod	
   points	
   to	
   oxidative	
   stress.	
   Mol	
   Cell	
   2011,	
  
          42,	
  561-­-568.	
  

     28.	
   Someya,	
  S.;	
  Yu,	
  W.;	
  Hallows,	
  W.C.;	
  Xu,	
  J.;	
  Vann,	
  J.M.;	
  Leeuwenburgh,	
  C.;	
  Tanokura,	
  M.;	
  Denu,	
  
          J.M.;	
   Prolla,	
   T.A.	
   Sirt3	
   mediates	
   reduction	
   of	
   oxidative	
   damage	
   and	
   prevention	
   of	
   age-­-
          related	
  hearing	
  loss	
  under	
  caloric	
  restriction.	
  Cell	
  2010,	
  143,	
  802-­-812	
  	
  

     29.	
   	
   	
   	
   Schlicker,	
   C.;	
   Gertz,	
   M.;	
   Papatheodorou,	
   P.;	
   Kachholz,	
   B.;	
   Becker,	
   C.F.;	
   Steegborn,	
   C.	
  
          Substrates	
   and	
   regulation	
   mechanisms	
   for	
   the	
   human	
   mitochondrial	
   sirtuins	
   Sirt3	
   and	
  
          Sirt5.	
  J	
  Mol	
  Biol	
  2008;	
  382,	
  790-­-801.	
  

     30.	
  	
  	
  	
  Shimada,	
  K.;	
  Nishikimi,	
  T.;	
  Kawarabayashi,	
  T.;	
  Takeuchi,	
  K.;	
  Takeda,	
  T.	
  Effect	
  of	
  prolonged	
  
          beta-­-adrenergic	
   blockade	
   induced	
   by	
   atenolol	
   on	
   left	
   ventricular	
   remodeling	
   after	
   acute	
  
          myocardial	
  infarction	
  in	
  the	
  rat.	
  Jpn	
  Heart	
  J	
  1995;	
  36,	
  81-­-9	
  

     31.	
   	
   	
   	
   Mela,	
   L.;	
   Seitz,	
   S.	
   Isolation	
   of	
   mitochondria	
   with	
   emphasis	
   on	
   heart	
   mitochondria	
   from	
  
          small	
  amounts	
  of	
  tissue.	
  Methods	
  Enzymol	
  1997;	
  55,	
  39-­-46	
  

     32.	
   	
   	
   	
   Barja,	
   G.	
   The	
   quantitative	
   measurement	
   of	
   H2O2	
   generation	
   in	
   isolated	
   mitochondria.	
   J	
  
          Bioenerg	
  Biomembr	
  2002;	
  34,	
  227-­-233	
  

     33.	
   	
   	
   Latorre,	
   A.;	
   Moya,	
   A.;	
   Ayala,	
   A.	
   Evolution	
   of	
   mitochondrial	
   DNA	
   in	
   Drosophila	
   suboscura.	
  
          Proc	
  Natl	
  Acad	
  Sci	
  USA	
  1986;	
  83,	
  8649-­-8653	
  

     34.	
  	
  	
  	
  Asuncion,	
  J.C.;	
  Millan,	
  A.;	
  Pla,	
  R.;	
  Bruseghini,	
  L.;	
  Esteras,	
  A.,	
  Pallardo,	
  F.V.;	
  Sastre,	
  J.;	
  Viña,	
  J.	
  
          Mitochondrial	
   glutathione	
   oxidation	
   correlates	
   with	
   age-­-associated	
   oxidative	
   damage	
   to	
  
          mitochondrial	
  DNA.	
  FASEB	
  J	
  1996;	
  10,	
  333-­-338	
  

     35.	
   	
   	
   Loft,	
   S.;	
   Poulsen,	
   H.E.	
   Markers	
   of	
   oxidative	
   damage	
   to	
   DNA:	
   Antioxidants	
   and	
   molecular	
  
          damage.	
  Methods	
  Enzymol	
  1999;	
  300,	
  166-­-184	
  

     36.	
   Pamplona,	
   R.;	
   Portero-­-Otin,	
   M.;	
   Ruiz,	
   C.;	
   Gredilla,	
   R.;	
   Herrero,	
   A.;	
   Barja	
   G.	
   Double	
   bond	
  
          content	
   of	
   phospholipids	
   and	
   lipid	
   peroxidation	
   negatively	
   correlate	
   with	
   maximum	
  
          longevity	
  in	
  the	
  heart	
  of	
  mammals.	
  Mech	
  Ageing	
  Dev	
  1999;	
  112,	
  169–183	
  

     37.	
   Rehsia,	
   N.S.;	
   Dhalla	
   N.S.	
   Mechanism	
   of	
   the	
   beneficial	
   effects	
   of	
   beta-­-adrenoceptor	
  
          antagonists	
  in	
  congestive	
  heart	
  failure.	
  Exp	
  Clin	
  Cardiol	
  2010;	
  15,	
  86-­-95	
  

     38.	
   	
   	
   	
   Sanchez-­-Roman,	
   I.;	
   Gomez,	
   J.;	
   Naudi,	
   A.;	
   Ayala,	
   V.;	
   Portero-­-Otín,	
   M.;	
   Lopez-­-Torres,	
   M.;	
  
          Pamplona,	
   R.;	
   Barja,	
   G.	
   The	
   beta-­-blocker	
   atenolol	
   lowers	
   the	
   longevity-­-related	
   degree	
   of	
  
          fatty	
   acid	
   unsaturation,	
   decreases	
   protein	
   oxidative	
   damage,	
   and	
   increases	
   extracellular	
  
          signal-­-regulated	
   kinase	
   signaling	
   in	
   the	
   heart	
   of	
   C57BL/6	
   mice.	
   Rejuv	
   Res	
   2010;	
   13,	
   683–
          693	
  

                                                                                                                            	
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