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ENRIQUE J. DE LA ROSA Y COLS. ANAL. REAL ACAD. NAC. FARM.

FIGURE 7.- Effect of caspase inhibition on the generation of RGCs. HH17
chick embryos were treated in ovo for 6 h with either vehicle (Control; A and C)
or with the broad-range caspase inhibitor Boc-D-fmk (Boc; B and D). Following

treatment, whole-mount retinas were stained for TUNEL (green in A-D) to
visualize apoptotic cells, and Islet 1/2 (red in C, D) to identify RGCs. (C)
and (D) correspond to higher magnification fields, where the increase in the
number of Islet 1/2 positive cells can be observed [reproduced with permission
from (24); © 2003 Federation of European Neuroscience Societies]. Calibration

bar, 200 µm (A, B) and 50 µm (C, D).

In addition, since deregulated cell death seems to be associated
with the progress of neurodegenerative disorders, new findings on
the regulation of physiological cell death during neural development
may be extrapolated to pathological conditions (31-34). Indeed, a

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