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VOL 66 (4) 2000 IN VITRO INDUCTION OF APOPTOSIS BY CYCLOSPORINE A

significant differences versus the control group are expressed as *P0.05, **P0.01
and ***P0.001.

Fig. 9: Effect of caspase inhibitors on CsA-induced chromatin condensation and
fragmentation. Hepatocytes were preincubated 1 hour with 100 µM Ac-YVAD-
AMC, Ac-DEVD-CHO and Ac-VEID-CHO before adding CsA in combination with
the inhibitors.
A: Chromatin condensation after treatment with 0 and 50 µM CsA after 4 hours
B: Chromatin fragmentation after treatment with 0 and 50 µM CsA after 4 hours
C: Chromatin condensation after treatment with 0, 10 and 25 µM CsA after 20 hours
D: Chromatin fragmentation after treatment with 0, 10 and 25 µM CsA after 20
hours
Data are expressed as mean r SD (n=3). Statistically significant differences compared
with 0 µM CsA are expressed as *P0.05, **P0.01 and ***P0.001. Statistically

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