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ANALES?DE?LA?REAL?ACADEMIA?NACIONAL?DE?FARMACIA? Article

ISSN (Online) 1697-4298 analesranf.com

Functional characterization of P2Y1 and P2X4 receptors in human
neuroblastoma SK-N-MC cells

Title in Spanish: Caracterización funcional de receptores P2Y1 y P2X4 en células SK-N-MC de neuroblastoma
humano

Javier Gualix1*, Miriam León-Otegui1, María Recuero2, María Jesús Bullido2, Fernando Valdivieso2
and M. Teresa Miras-Portugal1

1Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. 2Centro
de Biología Molecular “Severo Ochoa” (C.S.I.C.-U.A.M.). Universidad Autónoma de Madrid, 28049, Madrid, Spain.
Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Madrid, Spain. IdiPAZ

- Instituto de Investigación Hospital Universitario La Paz, Madrid, Spain

ABSTRACT: Nucleotides are important signalling RESUMEN: Los nucleótidos son importantes
molecules in both the peripheral and central nervous moléculas señalizadoras en el sistema nervioso. El
system. However, the in vitro study of their receptors estudio in vitro de sus receptores puede verse
can be hampered by the heterogeneity of primary obstaculizado por la heterogeneidad de los cultivos
neuronal cultures. The use of clonal neuroblastoma cell neuronales. El uso de líneas celulares de neuroblastoma
lines allows to circumvent this difficulty, so these lines permite eludir esta dificultad y dichas líneas se utilizan
are often used as a model to analyze the properties, frecuentemente como un modelo con el que analizar las
regulation and physiological role of nucleotide receptors propiedades, regulación y función de los receptores de
in neural tissues. Expression studies indicated the nucleótidos en tejidos neurales. Estudios de expresión
uFPprpu2eoXnsnce5tn,iocaenPpa2polXlifca6anPta2ialoYnynds1e,soPPf2s2hXYAo76wD, epPPdr,2oYttre2a1i-1nnM,ssPieei2SnnYAt 1DS[3C,KPa-P2N2+o]-XriM1inAC, cDPrec2PaeXßslleS4ss.,. SPin2KdY-icN1a3-,rMoPn2CXl.a1Ap,nrPeá2sliXesni4sc,ifaPu2ndXcei5op,nrPoatl2eeXsín6masyoPsPt22rYaXr1o7,nePni2nYlcar6se, mcPé2elnYutl1oa1ss,
transitorios odeA[DCPaß2+S],i tras la aplicación de ADP, 2-
Responses to these agonists seem to be mediated by a MeSADP respuestas que parecen estar
iMbPnl2ocRYrcSek1a2ars1dee7cese9p.ientoxAreS,rTKtaeP-sdNd-weMbmayCsontcsahetlerlsalost.ePd2RaYbbelsy1e-pstoehtnleoesceatsliimnvtdeoouscAtaencTotPam[gCwopanl2eei+rts]eeti mediadas a través ubnlorqeuceeoptocrasPi2Yto1t,alcoemjeorcsideopopnoer de
manifiesto por el el
aisninedtnaudgjoooniinssctuaresmereleensctptouisveosdteaP2[CYpa1a,2r+Mc]ii aRelnmS2ela1ns7te9c.élEbullloaAsqTuSePKadt-aNam-MbpiéConr,
partially blocked by MRS2179 and the P2X antagonist MRS2179 y por el antagonista P2X TNP-ATP, lo que
TNP-ATP, thus suggesting that ATP can interact with
two different P2 receptors: a Pse2nYsi1tirveecePp2toXr, inhibited by sugiere que el ATP puede interactuar con dos receptores
MRS2179, and a TNP-ATP receptor. To P2 diferentes: uPn2Xrecseepntsoibr lPe2aYT1,NinPh-iAbTidPo. por MRS2179,
characterize the P2X receptor responsible for the y un receptor Se caracterizó
MRS2179-resistant component of the ATP response, we el receptor P2X analizando el efecto de varios agonistas
BwCanezhalAellynsTzPdeci,detahlllnestohetowfusfgeehrchoetw[oCcfrhae2sas+epl]lvoieennirsngaecelsrdePta2oswXeeistiahtchgoeouCrnliTdasP,tßsb.-emoBneooAb[tsChTeaPr2vt+heo]deir. emneAlaT[PCoa2B+]zi.ANTiPn,gauunnaquceélsuelaobmsoersvtraórornesipnucreesmtaesnatoas ,dße-
T[Caan2t+o]i cuando las células fueron estimuladas con CTP.
response to CTP and the MRS2179-resistant component la respuesta a CTP como el componente de la
respuesta a ATP resistente a MRS2179, se potenciaron
of ATP response were potentiated by ivermectin. Such en presencia de ivermectina. Todos estos datos sugieren
pharmacological profile is consistent with the presence la presencia de un receptor P2X4 funcional en las
of a functional P2X4 receptor in SK-N-MC cell line. células SK-N-MC.
.

*Corresponding Author: jgualix@ucm.es An Real Acad Farm Vol. 81, Nº 3 (2015), pp. 247-257
Received: June 22, 2015 Accepted: November 5, 2015
Language of Manuscript: English

1. INTRODUCTION differentiation, proliferation, migration and death (1, 2).
Nucleotide-mediated signaling mechanisms and specific
Extracellular nucleotides have major roles in the receptor subtypes have been shown to be involved in
activity of neural cells. They act as transmitters or various pathological conditions including brain trauma and
modulators in most nerve types in the peripheral and ischaemia, neurodegenerative diseases involving
central nervous system. Furthermore, they also act as neuroimmune and neuroinflammatory reactions, as well as
important mediators in neuronal-glia or glia-glia in neuropsychiatric diseases (3).
communication and are trophic factors participating in cell
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@Real Academia Nacional de Farmacia. Spain