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SILENCING$BETA2$ADRENERGIC$RECEPTORS…$$
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3.2.$Demonstration$of$the$beta2$receptor$silencing$
When! comparing! the! fluorescence! level! of! nonYtreated! cells! with! those!
treated! with! the! siRNA! designed! for! the! beta2! adrenergic! receptor,! it! was! clear!
that!the!siRNA!was!able!to!significantly!reduce!the!amount!of!receptors!according!
to! the! clear! fading! of! the! fluorescence! present! in! treated! cells.! As! observed! in!
Figure! 2,! there! was! a! reduction! in! the! presence! of! the! beta2! receptors! in! the!
treated! ciliary! nonYpigmented! epithelial! cells! which! reduction! was! 66.5! ±! 7.2! %!
with!respect!to!control!(100!%;!n=4).!
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Figure.2.7!Beta2!adrenergic!immunostaining!of!ciliary!nonYpigmented!epithelial!cells!before!(upper!
panels)!and!after!!the!treatment!of!the!siRNA!(lower!panels).!The!receptor!can!be!observed!in!green!
on!the!left!panels!while!Nomarsky!Interferencial!Contrast!can!observe!the!same!cells!on!the!right.!
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3.3.$Commercial$drugs$
The! validation! of! our! model! was! made! using! a! battery! of! ophthalmic!
commercial!drugs!on!New!Zealand!White!rabbits.!In!these!studies!the!activities!on!
IOP! of! Trusopt! (dorzolamide),! Xalatan! (latanoprost)! and! Timoftol! (timolol)! have!
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