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JESÚS%PINTOR%

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been! assayed.! A! single! drop! (40! µL)! has! been! instilled! in! rabbit! eyes! and! IOP!
measures!have!been!performed!each!hour!for!8!hours.!!

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Figure. 3.7! Comparative! effects! of! commercial! compounds! currently! used! for! the! treatment! of!
glaucoma!as!well!as!the!siRNA!used!along!this!research.!(***p<0.001).!

!
As! described! in! Figure! 3,! any! of! those! compounds! produced! a! reduction! in!

IOP! of! between! 20Y35%! and! the! duration! of! this! effect! was! about! 6! hours.! These!
experiments! confirmed! the! suitability! of! this! animal! model,! due! to! its! ability! and!
flexibility!to!regulate!the!IOP,!to!be!used!with!RNAi!applications.!!
3.4.$Side$effects$

The! integrity! of! the! ocular! structures! after! the! siRNAs! application! was!
assessed! observing! the! ciliary! processes! under! microscopy! by! means! of!
haematoxylinYeosin! staining! (Figure! 4).! No! differences! were! observed! between!
treated!and!nonYtreated!animals.!
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236%

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