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3.(RESULTS(
3.1.$Effect$of$beta2$adrenergic$receptor$silencing$

To! see! whether! the! silencing! of! beta2! adrenergic! receptors! produced!
changes! in! the! IOP,! the! rabbits! treated! with! the! siRNA! against! the! beta2! receptor!
were!monitored!in!their!IOP!for!7!days.!As!presented!in!figure!1,!there!was!a!robust!
reduction! in! IOP! which! started! to! be! measurable! 24! hours! after! the! instillation! of!
the!siRNA!and!which!was!statistically!significant!for!5!days.!The!maximal!reduction!
obtained! after! silencing! the! beta2! receptor! was! 30! ±! 5! %! compared! to! control!
(n=6).! This! and! similar! values! were! maintained! for! 1Y2! days,! with! the! IOP!
remaining! low! for! three! more! days! (with! a! mean! of! 30! %! of! reduction)! and! then!
returning!slowly!towards!control!values!(Figure!1).!!
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Figure. 1.7! Effect! of! a! siRNA! against! the! beta2! adrenergic! receptor.! Intraocular! pressure! was!
reduced! 24! hours! after! the! treatment! started! returning! to! normal! values! almost! 5! days! after! the!
treatment!started.!The!scrambled!siRNA!did!not!significantly!modify!intraocular!pressure.!

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In! order! to! further! confirm! the! selectivity! of! the! tested! siRNA,! a! scramble!
siRNA! was! also! assayed.! As! observed! in! Figure! 1! after! the! treatment! with! the!
scramble! siRNA,! rabbit! IOP! did! not! significantly! change! during! the! time! of! the!
experiment.!!
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