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VOL. 76 (3), 357-377, 2010 TSC1-TSC2 COMPLEX ON THE CROSSROAD OF PANCREATIC...

Figure 4. Effect of TSC2 interference on insulin and glucose signaling. A) 24
h after siRNA transfection cells were stimulated with insulin 10 nM for 5 min (IR
+/+, Rec A and Rec B), or glucose 5 mM for 15 min (in IR -/- cells, right upper pan-
nel). Specific inhibitors were added 30 min before stimulation. Blots are representa-
tive of four independent experiments. B) Densitometric analysis showing the percent-
age of TSC2 deletion in TSC2-interfered cells compared to control cells. Results are
means ± S.E.M. of the level of interference reached. C) Fold-increase of p70S6K Thr389
phosphorylation after insulin or glucose stimulation in control and TSC2-interfered
cells. Results are means ± S.E.M. *P < 0.05 compared to control cells. The differences
between the ratios of fold-stimulation between control and TSC2-interfered cells were
calculated and significant differences are indicated #P < 0.05. D) Fold-increase of Akt
Ser473 phosphorylation after insulin or glucose stimulation in control and TSC2-in-
terfered cells. Results are means ± S.E.M. *P < 0.05 compared to control cells.

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