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An. R. Acad. Nac. Farm., 2010, 76 (1): 3-22

ARTÍCULO

Cross-talk between glutamate and nucleotide
receptors in cerebellar granule neurons
in culture

Elvira Salas 1, 3, David León Navarro 1, 2,
Jesús Sánchez-Nogueiro 1, Patricia Marín-García 1,
María Teresa Miras-Portugal 1 *

1 Department of Biochemistry and Molecular Biology IV.
Faculty of Veterinary. Complutense University of Madrid. Spain.
2 Biochemistry Area. Chemistry Faculty. Castilla La Mancha University.
Spain.
3 Department of Biochemistry. Medicine Faculty. Costa Rica University.
Costa Rica.
Recibido el 18 de septiembre de 2009.

ABSTRACT

ATP elicits Ca2+ transients in cultured cerebellar granule neurons
acting through specific ionotropic (P2X) and metabotropic (P2Y)
purinergic receptors. In these neurons, application of L-Glutamate
(L-Glu) immediately before ATP induced a prolonged reduction
of ATP-mediated responses that remains at least 5 minutes after
L-Glu wash out. alpha-amino-3-hydro-5-methyl-4-isoxazolpropionic
acid (AMPA), N-methyl-D-aspartate (NMDA) and 3,5-dihydro-
xyphenyl-glycine (DHPG), selective agonists of ionotropic non-NMDA,
NMDA and Group I metabotropic glutamate receptors respectively,
mimicked Glu-induced attenuating effects. The activity of calcium-
calmodulin dependent protein kinase II (CaMKII) seems to be
involved, at least at long term, because inhibitors of CaMKII, 1-[N,O-
bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4phenylpiperazine

3