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VOL. 76 (1), 23-44, 2010 BETA-CELL HYPERPLASIA INDUCED BY HEPATIC INSULIN...
3.3. Progressive beta-cell hyperplasia and failure of insulin
3.3. secretion with aging
iLIRKO showed progressive beta-cell hyperplasia as compar-
ed with controls. More importantly, the level of hyperplasia was co-
rrelated to the level of IR expression remaining in the liver (Figure 6,
panel B). The iLIRKO mice also developed progressive hyperinsuline-
mia as compared with controls and paralleled with the level of IR
deletion by the liver (Figure 6, panel B). This beta-cell expansion per-
sisted throughout life as shown in Figure 6, panel D. In parallel, plas-
Figure 4. Generalized Insulin Resistance persists in aged iLIRKO. In vivo
insulin signaling studies were performed in 1-year-old Control and iLIRKO mice by
intraperitoneal injection of 1 U/kg body weight of human insulin. After 10 minutes,
the tissues were removed and analyzed by Western blot. A representative experi-
ment out of four is shown. Histograms show the densitometric analysis.
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