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ÓSCAR ESCRIBANO Y COLS. AN. R. ACAD. NAC. FARM.
reported in early insulin IR deletion (8). However, no effect was
observed on phosphoenol pyruvate carboxykinase (PEPCK) or fatty
acid synthase (FAS) gene expression in 6 month-old iLIRKO mice as
compared with controls (Figure 2, lower panels).
Figure 1. Induction of Liver-Specific IR Knockout. A) (Upper panel) Liver
protein extracts from 6-month-old Control and iLIRKO mice were analyzed by
Western blot. (Lower panel) The autoradiograms corresponding to four independent
experiments were quantitated by scanning densitometry. B) Protein extracts of
several tissues from 6-month-old Wt, IR(loxP/loxP) and iLIRKO mice were analyzed
by Western blot. A representative experiment out of four is shown. Results are
expressed as mean +/– SEM. **P < 0.005; *P < 0.05; iLIRKO vs. Control.
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