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125ANALESRANFwwwanalesranfcomAntibioticresistanceininfectionsassociatedwithhealthcareinCOVID-19pandemicJorgeAAlmeidaVillegasJoséAGutiérrezGutiérrezetalAnRealAcadFarmVol88nº2(2022)·pp123-130Bacterialgrowthandsusceptibilitytestingbronchialsecretionswereobtainedbythetrachealaspiration techniquein sterilebronchialaspiration trapsby themedicalornursingstaffthebronchialsecretionswereanalyzedintheclinicallaboratory,in themicrobiologyarea they wereprocessedinabiosafetycabinetspreadsofthesamplewerepreparedonaslidetoperform theGram stain for theacceptanceof the sample withanalyticalquality, theMurray-Washingtoncriteria wereapplied(10) whichestablishtheacceptablequalityforcultureofthesampleifithasgreaterthan25leukocytesperfieldandlessthan(10)squamousepithelialcellsper fieldin theobservation with theobjectiveof100Xsampleswithgreaterthanorequalto(10)epithelialcellsperfieldin100Xwererejectedtheculturefortherestofthesampleswascarriedoutbycross-streakingin 5% sheepsbloodagaragar,chocolateagaragarandMacconkeyagar,theywereincubatedat36+1ºcundermicroaerophilicconditionsfor24to48hoursbacterialidentificationwascarriedoutusingpanelnc86andPc33 forgramnegativebacilliand forgrampositivecoccirespectively. theidentificationandantibiogramof themicroorganismwascarriedoutinacommercialMicroscan4®system(beckmanUsA)theinterpretationoftheminimuminhibitoryconcentrationintermsofsensitivitywasusedbythereferencecriteriaofthecLsI(clinical&LaboratorystandardsInstitute)ForthedetectionofExtendedspectrumbetalactamasesreportedbytheteamaphenotypicmanualtestwasperformedTable1Demographicandclinicalcharacteristicsof theevaluatedpatientsn(%)(DE)Age(years)502(121)Malegender60(600)-Overweight32(320)Obesity29(290)-Diabetesmellitustype231(310)Arterialhypertension27(270)-Ischemicdisease5(50)-Immunosuppression0(00)-HIV0(00)-Positivesmoking31(310)-PcrPositive75(750)-negative3(30)-notconfirmed22(220)-IcU100(1000)-spO2>700(1000)-Empiricaltreatment100(1000)-Death100(1000)-HIVHumanImmunodeficiency VirusPcrPolymerasechainreactionIcUIntensivecareUnitspO2OxygensaturationEmpirical treatment;Levofloxacinand thirdgenerationcephalosporins