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241ANALESRANFwwwanalesranfcomBiotininterferenceinseveralelectrochemiluminescenceimmunoassays(eclia)JoséLuisMartín-CalderónAnRealAcadFarmVol87nº3(2021)·pp239-246theinterferent-spikedspecimenandC0istheconcentrationinthespecimen devoid ofinterferentInterference was considered significantwhen therelativebiasexceeded10%(9)Inaddition weperformedaGlickinterferographplottingthepercentageofchangevsbiotinconcentration3RESULTSIntable2weshowthedeviationoftheimmunoassaysresultsafterspiking with variousconcentrationsofbiotineachleveloftheconsideredconstituentAsexpectedtherewasastrongassociationbetweentheplasmabiotinconcentrationsandthedegreeofinterferenceinnearlyalltheimmunoassaysassessedFromthesedatawecanconcludethatresultswerespuriouslydecreasedin12sandwichimmunoassaysandfalselyincreasedin11competitiveimmunoassaysHowever, whenanalyzing thyrotropin(TSH)orCA153antigentheresultswerenotsignificantlyaffectedinanyofthethreelevelsofanalyteconcentrationassessed(datanotshownonthetable2)Fromdataanalysiswecaninferthatbiotininterferencewasnon-dependentofanalyteconcentrationforfreethyroxinfreeT3prolactinestradiolfolateprogesteronetestosteronealphafetoproteincarcinoembrionicantigenCA125CA19-9HE4antigenantithyroglobulinantibodiesorantithyroperoxidaseantibodiesOntheoppositethebiasduetobiotinwasanalyteconcentrationdependent forFSHLHprostate specificantigen freeprostate specificantigenvitaminB1225-hydroxyvitaminDparathyrinecortisolandC-terminalcollagentypeIpeptidesFigure1showsGlick´sinterferographsforfourdifferentpanelsthyroidbonemetabolismandvitaminstumormarkersandgonadalaxisForthesewehaveconsideredonlythemediumconcentrationofanalyteTable1Baselineconcentrationsofanalytes
