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Ana I. Arroba, Ángela M. Valverde

weeks (Figure 6C).

B

C

Figure 6. Circulating endotoxemia and pro-inflammatory cytokines during DR progression in db/db mice. A) Serum endotoxin in
db/db and db/+ mice at different ages. Results are means ± SEM (n=5 retinas per condition). *p=0.05 db/db vs db/+ matched at each age.
B) (left panel) M1 (iNOS) and M2 (arginase-1) mRNAs in db/db and db/+ mice at 5, 8 and 20 weeks. Results are expressed as 2-?Ct.
(right panel) iNOS and arginase-1 protein levels in retinas from db/db and db/+ mice analyzed by Western blot at the ages indicated.
Results are means ± SEM (n=5 retinas per condition). *p=0.05 db/db vs db/+ matched at each age. C) iNOS immunostaining (green)
counterstained with DAPI (blue) (n=5 retinas per condition). Retinal layers are labelled as ONL (outer nuclear layer), OPL (outer
plexiform layer), INL (inner nuclear layer), (IPL) inner plexiform layer and GCL (ganglion cell layer). At least 3 retinas and 4 non-
adjacent sections per retina were analyzed for each experimental condition.

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