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from k. senegalensis flowers. The method used enabled Elsayed A. Aboutabl et al.
identification of five flavonoid glycosides and twelve
limonoids of different types viz: mexicanolides, 8. Mosmann T. Rapid colourimetric assays for cellular
phragmalins and angolensate . Pathogens developed growth and survival: Application to proliferation and
resistance to antibiotics is one of very common health cytotoxicity assays. J Immunol Methods 1983; 65: 55-
problem for human infectious diseases. Being 63.
ethnomedicaly used for treating infection such as diarrhea,
wounds and syphilis the results deserve further 9. Thabrew, MI, Hughes RD, McFarlane IG. Screening
biochemical studies of extracts from the flowers for their of hepatoprotective plant components using a HEPG2
potential medicinal significance. cell cytotoxicity assay. J Pharm Pharmacol 1997; 49:
1132-5.
6. CONFLICT OF INTEREST
10. Niessen WMA, Tinke AP. Liquid chromatography–
The authors declare no conflict of interest. mass spectrometry. General principles and
instrumentation. J. Chromatogr A 1995; 703: 37–57.
7. ACKNOWLEDGMENTS
11. Markham KR. Techniques of Flavonoid Identification.
Authors are grateful to Dr. M. Farag for the UPLC-MS Academic Press: London. 1982.
analysis in (Leibniz Institute for Plant Biochemistry, Halle,
Germany). Special gratitude for Miss Therese Labib and 12. Mesbah MK, Abd El-Hafiz MA , El-Shanawany MA.
Dr. M.El-Gibali plant taxonomy consultants at the Pharmacognostic study of Khaya senegalensis A. Juss
Ministry of Agriculture, Giza, Egypt for authentication of growing in Egypt. 1: Botanical and chemical study of
the plant specimens. The work was funded by National the leaf. Bull Pharm Sci 1984; 7: 207-223.
Research Centre, Dokki, Giza, Egypt. Through a PhD
grant No. 2/5/10. 13. Liu EH, Qi LW, Ping Li JC, Li CY, Peng YB.
Advances of modern chromatographic and
8. REFERENCES electrophoretic methods in separation and analysis of
flavonoids. Molecules 2008;13: 2521-2544
1. Roy A, Saraf S. Limonoids: overview of significant
14. Nakatani M, Abdelgaleil SAM, Okamura H, Iwagawa
bioactive triterpenes distributed in plant kingdom. T, Sato, A, Doe M. B. Limonoids from an Egyptian
mahogany Khaya senegalensis. Tennen. Yuki
Biol Pharm Bull 2006; 29(2): 191-201 Kagobutsu Toronkai Koen Yoshishu 2000;42: 37-42.
2. Yang W, Fang D M, He HP, Hao XJ, Wu ZJ, Zhang 15. Olmo RV, Fatima M, Das GF, Da Silva Fo ER, Vieira
PC, Fernandes JB, Marsaioli AJ, Pinheiro AL , Vilela
GL. Analysis of mexicanolide- and phragmalin-type EF. Rearranged limonoids from Khaya senegalensis.
Phytochemistry 1996;42(3): 831-837.
limonoids from Heynea trijuga using high-
16. Nakatani M, Abdelgaleil SAM, Okamura H, Iwagawa
performance liquid chromatography/ electrospray T.; Sato A, Doe M. A Khayanolides A and B, new
rearranged phragmalin limonoid antifeedants from
tandem mass spectrometry. Rapid Commun. Mass Khaya senegalensis. Tetrahedron Let 2000; 41 (33):
6473-6477.
Spectrom 2013;27: 1203–1212 2 15.
17. Govindachari TR, Kumari GN. Tetraterpenoids from
3. Nikiema A, Pasternak D. Khaya senegalensis Khaya senegalensis. Phytochemistry 1998; 47(7):
1423-1425.
(Desr.)A.Juss. (Internet) Record from
18. Tchimene MK, Tane P, Ngamga D, Connolly JD,
PROTA4U.Louppe D , Oteng-Amoako, AA and Farrugia LJ. Four tetranortriterpenoids from the stem
bark of Khaya anthotheca, Phytochemistry 2005; 66:
Brink M (Editors).PROTA (Plant Resources of 1088-1093
Tropical Africa / Ressources végétales de 19. Tian Q, Miller EG, Ahmad H, Tang L, Patil BS.
Differential inhibition of human cancer cell
l’Afriquetropicale), Wageningen, 2008. Netherland. proliferation by citrus limonoids. Nutr Cancer 2001;40
(2):18.
Available from <http://www.prota4u.org/search.asp>.
20. Androulakis XL, Muga SJ, Chen F, Koita Y, Tour B,
Accessed 30 October 2014. Wargovich MJ. Chemopreventive Effects of Khaya
senegalensis Bark Extract on Human Colorectal
4. Adesida GA, Adesogan EK, Okorie DA, Taylor DAH, Cancer. Anticancer research 2006; 26: 2397-2406.
Styles BT. Limonoid chemistry of the genus Khaya 21. ElSouda S, Mammoun A, Hashem F, Aboutab EA. In
vitro cytotoxic activity of essential oil from khaya
(Meliaceae). Phytochemistry 1971; 10: 1845-53. species flowers.The 61st International Congress and
Annual Meeting of the Society for Medicinal Plant
5. Yuan CM, Zhang Y, Tang GH, Di YT, Cao MM, and Natural Product Research . 2013. Münster,
Germany, 1st – 5th September 2013 . Planta Med .
Wang XY, Zuo G, Li SL, Hua HM, He HP, Hao 2013;79 :P 136. DOI: 10.1055/s-0033-
1352126.Congress abstract.
XJ. Khayseneganins A–H, Limonoids from Khaya
22. Adebayo OL, Osman K. A comparative evaluation
senegalensis. J Nat Prod 2013; 76 (3): 327–333. of in vitro growth inhibitory activities of different
6. Wu Q, Wang M, Simon JE. Determination of @Real Academia Nacional de Farmacia. Spain
isoflavones in red clover and related species by high-
performance liquid chromatography combined with
ultraviolet and mass spectrometric detection. J
Chromatogr A 2003;1016: 195– 209.
7. Muanza DN, Kim BW , Euler KL , Williams L.
Antibacterial and Antifungal Activities of Nine
Medicinal Plants from Zaire. Int J Pharmacog
1994;32: 337.
262