Page 55 - 81_03
P. 55

from k. senegalensis flowers. The method used enabled                                                  Elsayed A. Aboutabl et al.
identification of five flavonoid glycosides and twelve
limonoids of different types viz: mexicanolides,               8. Mosmann T. Rapid colourimetric assays for cellular
phragmalins and angolensate . Pathogens developed                   growth and survival: Application to proliferation and
resistance to antibiotics is one of very common health              cytotoxicity assays. J Immunol Methods 1983; 65: 55-
problem for human infectious diseases. Being                        63.
ethnomedicaly used for treating infection such as diarrhea,
wounds and syphilis the results deserve further                9. Thabrew, MI, Hughes RD, McFarlane IG. Screening
biochemical studies of extracts from the flowers for their          of hepatoprotective plant components using a HEPG2
potential medicinal significance.                                   cell cytotoxicity assay. J Pharm Pharmacol 1997; 49:
                                                                    1132-5.
6. CONFLICT OF INTEREST
                                                               10. Niessen WMA, Tinke AP. Liquid chromatography–
The authors declare no conflict of interest.                        mass spectrometry. General principles and
                                                                    instrumentation. J. Chromatogr A 1995; 703: 37–57.
7. ACKNOWLEDGMENTS
                                                               11. Markham KR. Techniques of Flavonoid Identification.
    Authors are grateful to Dr. M. Farag for the UPLC-MS            Academic Press: London. 1982.
analysis in (Leibniz Institute for Plant Biochemistry, Halle,
Germany). Special gratitude for Miss Therese Labib and         12. Mesbah MK, Abd El-Hafiz MA , El-Shanawany MA.
Dr. M.El-Gibali plant taxonomy consultants at the                   Pharmacognostic study of Khaya senegalensis A. Juss
Ministry of Agriculture, Giza, Egypt for authentication of          growing in Egypt. 1: Botanical and chemical study of
the plant specimens. The work was funded by National                the leaf. Bull Pharm Sci 1984; 7: 207-223.
Research Centre, Dokki, Giza, Egypt. Through a PhD
grant No. 2/5/10.                                              13. Liu EH, Qi LW, Ping Li JC, Li CY, Peng YB.
                                                                    Advances of modern chromatographic and
8. REFERENCES                                                       electrophoretic methods in separation and analysis of
                                                                    flavonoids. Molecules 2008;13: 2521-2544
1. Roy A, Saraf S. Limonoids: overview of significant
                                                               14. Nakatani M, Abdelgaleil SAM, Okamura H, Iwagawa
bioactive triterpenes distributed in plant kingdom.                 T, Sato, A, Doe M. B. Limonoids from an Egyptian
                                                                    mahogany Khaya senegalensis. Tennen. Yuki
Biol Pharm Bull 2006; 29(2): 191-201                                Kagobutsu Toronkai Koen Yoshishu 2000;42: 37-42.

2. Yang W, Fang D M, He HP, Hao XJ, Wu ZJ, Zhang               15. Olmo RV, Fatima M, Das GF, Da Silva Fo ER, Vieira
                                                                    PC, Fernandes JB, Marsaioli AJ, Pinheiro AL , Vilela
GL. Analysis of mexicanolide- and phragmalin-type                   EF. Rearranged limonoids from Khaya senegalensis.
                                                                    Phytochemistry 1996;42(3): 831-837.
limonoids from Heynea trijuga using high-
                                                               16. Nakatani M, Abdelgaleil SAM, Okamura H, Iwagawa
performance liquid chromatography/ electrospray                     T.; Sato A, Doe M. A Khayanolides A and B, new
                                                                    rearranged phragmalin limonoid antifeedants from
tandem mass spectrometry. Rapid Commun. Mass                        Khaya senegalensis. Tetrahedron Let 2000; 41 (33):
                                                                    6473-6477.
Spectrom 2013;27: 1203–1212 2 15.
                                                               17. Govindachari TR, Kumari GN. Tetraterpenoids from
3. Nikiema A, Pasternak D. Khaya senegalensis                       Khaya senegalensis. Phytochemistry 1998; 47(7):
                                                                    1423-1425.
(Desr.)A.Juss.  (Internet)  Record               from
                                                               18. Tchimene MK, Tane P, Ngamga D, Connolly JD,
PROTA4U.Louppe D , Oteng-Amoako, AA and                             Farrugia LJ. Four tetranortriterpenoids from the stem
                                                                    bark of Khaya anthotheca, Phytochemistry 2005; 66:
Brink M (Editors).PROTA (Plant Resources of                         1088-1093

Tropical Africa / Ressources végétales de                      19. Tian Q, Miller EG, Ahmad H, Tang L, Patil BS.
                                                                    Differential inhibition of human cancer cell
l’Afriquetropicale), Wageningen, 2008. Netherland.                  proliferation by citrus limonoids. Nutr Cancer 2001;40
                                                                    (2):18.
Available from <http://www.prota4u.org/search.asp>.
                                                               20. Androulakis XL, Muga SJ, Chen F, Koita Y, Tour B,
Accessed 30 October 2014.                                           Wargovich MJ. Chemopreventive Effects of Khaya
                                                                    senegalensis Bark Extract on Human Colorectal
4. Adesida GA, Adesogan EK, Okorie DA, Taylor DAH,                  Cancer. Anticancer research 2006; 26: 2397-2406.

Styles BT. Limonoid chemistry of the genus Khaya               21. ElSouda S, Mammoun A, Hashem F, Aboutab EA. In
                                                                    vitro cytotoxic activity of essential oil from khaya
(Meliaceae). Phytochemistry 1971; 10: 1845-53.                      species flowers.The 61st International Congress and
                                                                    Annual Meeting of the Society for Medicinal Plant
5. Yuan CM, Zhang Y, Tang GH, Di YT, Cao MM,                        and Natural Product Research . 2013. Münster,
                                                                    Germany, 1st – 5th September 2013 . Planta Med .
Wang XY, Zuo G, Li SL, Hua HM, He HP, Hao                           2013;79 :P 136. DOI: 10.1055/s-0033-
                                                                    1352126.Congress abstract.
XJ. Khayseneganins A–H, Limonoids from Khaya
                                                               22. Adebayo OL, Osman K. A comparative evaluation
senegalensis. J Nat Prod 2013; 76 (3): 327–333.                     of in vitro growth inhibitory activities of different

6. Wu Q, Wang M, Simon JE. Determination of                             @Real Academia Nacional de Farmacia. Spain

isoflavones in red clover and related species by high-

performance liquid chromatography combined with

ultraviolet and mass spectrometric detection. J

Chromatogr A 2003;1016: 195– 209.

7. Muanza DN, Kim BW , Euler KL , Williams L.

Antibacterial and Antifungal Activities of Nine

Medicinal Plants from Zaire. Int J Pharmacog

1994;32: 337.

262
   50   51   52   53   54   55   56   57   58   59   60