Page 106 - 79_04

Alexia
Gómez
&
col.

The
fatty
acid
composition
of
heart
mitochondria
lipids
and
derived
indexes

were
also
measured
(Table
4).
The
fatty
acids
18:0,
20:4n-­-6,
22:0,
22:4n-­-6,
22:5n-­-
6,
22:5n-­-3,
22:6n-­-3
and
24:0
were
significantly
lower
and
the
fatty
acid
18:2n-­-6

was
significantly
higher
in
the
atenolol
group.
Due
to
these
changes
the
acyl
chain

length
decreased
(ACL;
0.66%
total
decrease),
the
PUFAn-­-3
strongly
decreased

(27.61%
total
decrease)
and
the
PUFAn-­-6
increased
(12.31
%
total
increase).
The

final
result
was
a
strong
decrease
in
the
peroxidizability
index
(PI;
15.20%
total

decrease)
and
a
non-­-significant
trend
to
decrease
the
total
number
of
double

bonds

(6.94%
total
decrease
in
the
double
bond
index,
DBI)
in
the
atenolol
group

in
relation
to

the
control
group
(P<0.01
in
PI;
Figure
3).

The
estimation
of
the
desaturase
and
elongase
activities
is
shown
in
Tables

5
and
6.
The
desaturase
activity
?9
(n-­-9)
was
higher,
while
?5
(n-­-6)
was
lower
in

the
atenolol
group.
Integrated
n-­-6
and
n-­-3
desaturation
and
elongation
activities

were
also
lower
in
the
atenolol
group.
The
elongase
activities
ELOVL
1/3,
ELOVL
5

(n-­-6),
ELOVL
2/5
(n-­-6)
and
ELOVL
2/5
(n-­-3)
were
significantly
lower
in
the

atenolol
group.

Figure
3.-­-
PI
and
DBI
(fatty
acid
unsaturation
degree)
and
total
PUFAn-­-6
and
PUFAn-­-3
of

heart
mitochondria
from
control
and
atenolol
treated
Wistar
rats.
PUFA=
polyunsaturated

fatty
acid;
DBI=double
bond
index;
PI=
Peroxidizability
index.
Abbreviations
and
calculations
of

fatty
acid
classes
and
indexes
are
explained
in
the
Materials
and
Methods
section.
Control
values:

116.22±5.49
(PI);
131.93±4.70
(DBI);
and
7.10±0.39
(for
PUFAn-­-3)
and
25.01±0.97
(for
PUFAn-­-6),

both
expressed
as
mol
%.
Values
are
means
±
SEM
from
8
different
samples
per
group.
Asterisks

represent
significant
differences
compared
to
the
control
group:
*
P<0.05;
**
P<0.01;
***P<0.001.

622