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ANTONIO L. DOADRIO Y COLS. AN. R. ACAD. NAC. FARM.

The aqueous complex was prepared extemporarely at room
temperate yielding 0.66 M cefotaxime-Cu final concentration. The
assays were done with this concentration and four other more diluted
in water.

3. RESULTS AND DISCUSSION

3.1. Concentration of cefotaxime

The concentration homogenates of free cefotaxime was measured
in blood, liver, kidney, spleen, lung and heart of the killed rats from
groups A and B at the 30, 60, 90, 120, 150, 180 and 210 min. after
the administration of cefotaxime. As there were 5 rats by each time,
the average concentration of free cefotaxime was calculated by each
group of 5 rats that were killed at each time.

Free antibiotic was present in all of samples showing a peak after
30 min of the administration the highest concentration of cefotaxime
was found in blood (four order of magnitude more cefotaxime than
the solid organs).

After the first 30 min, the antibiotic concentration dropped from
the maximum to the minimal values reached after 3 hours and half
of the beginning of the experiment.

The declining plots depicted a «two-phase» kinetic, inespectively
of the group of rats or the organ source of the samples, in good
agreement with the conclusion of the decrease of free antibiotic
found in adult humans with normal renal function (11, 12).

In the first 30-90 min cefotaxime disappeared from the sample
slowly, afterwards the declining late accelerated.

We can establish conclusive results describing that the amount of
antibiotic is much higher in control rats than in copper-toxified rats.

The differences more significant have been seen in liver, kidney
and lung. In spleen and heart, those differences were less prominent,
although they are very appreciable. In blood, concentrations of free
cefotaxime were greater in rats without copper, but the differences
between concentrations are not as rough as in the others samples

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