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An. R. Acad. Nac. Farm., 2007, 73 (4): 901-925

Revisión

Roles of Protein Phosphatase Type 1 in
Schizosaccharomyces pombe

Recibido el 4 de octubre de 2007

ISABEL ÁLVAREZ-TABARÉS1-3, IAIN M. HAGAN2 AND JOSÉ-
MIGUEL ORTIZ MELÓN1*

1Departamento de Biología Molecular, Facultad de Medicina, Unidad
de Biomedicina-CSIC, Universidad de Cantabria, 39011 Santander,

Spain.
2CRUK Cell Division Group, Paterson Institute for Cancer Research,
University of Manchester, Wilmslow Road, Manchester M20 4BX, UK.
3Present address: Departamento de Biotecnología Microbiana, Centro

Nacional de Biotecnología-CSIC, Campus de Cantoblanco-UAM,
28049 Madrid, Spain.

ABSTRACT

Protein phosphatases are considered to be global regulators in many biological
processes. They are divided in families on the basis of substrate specificity,
mechanisms of catalysis and evolutionary relations. Protein Phosphatase Type 1
(PP1) holoenzymes are composed of a small number of catalytic subunits and an
array of regulatory, targeting, subunits. The S. pombe genome encodes two highly
related catalytic subunits, Dis2 and Sds21. We fused enhanced green fluorescence

* Correspondence: José-Miguel Ortiz Melón. Departamento de Biología Molecular,
Facultad de Medicina, Unidad de Biomedicina-CSIC, Universidad de Cantabria, 39011
Santander. Tel. 942202941. Fax: 942201945.

e-mail: ortizjm@unican.es
Abbreviations: PP, Protein Phosphatase; S. pombe, Schizosaccharomyces pombe;
EGFP, Enhanced Green Fluorescent Protein; RNA, Ribonucleic Acid; RNAi, Interfering
RNA; Dis, Defective in sister chromatid disjoining; Tyr, Tyrosine; PTP, Protein Tyro-
sine Phosphatase; Asp, Aspartic Acid; Ser, Serine; Thr, Threonine; S. cerevisiae, Sac-
charomyces cerevisiae; A. thaliana, Arabidopsis thaliana; D. melanogaster, Drosophila
melanogaster; C. elegans, Cenorabditis elegans; DNA, Deoxyribonucleic Acid; BIM, Blo-
ked in mitosis; GFP, Green Fluorescent Protein.

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