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FLORA DE PABLO Y COLS. AN. R. ACAD. NAC. FARM.

FIGURE 2. Distribution of factors by immunofluorescence in chick embryos. A-D) E1
embryos (stage 6), E-H) E2.5 embryos (stage 15). Transversal criosections at mid-
level in A, and caudal to the viteline artery in E (microscopy taken from Bellairs and
Osmond, The Atlas of Chick Development. 1998. San Diego, California, Academic
Press). Proinsulin is detected with an antipeptide-C antiserum (Rb40) in B (inset
shows a detail with labelled neuroectodermic cells in the lateral zone, arrowheads)
and F (positive areas include the floor plate of the neural tube, notochord and der-
mamyotome of somite). IGF-I immunostain is negative in C and labelled only a few
ectodermic (short arrow) and digestive endodermic cells (long arrows) in G. IGF-II
immunostain labelled basal membranes in D (arrow) and H (note around notochord
and neural tube). Fluorochorome was Cy2 and images were captured with a CCD
camera. ec, ectoderm; m, mesoderm; en, endoderm; n, notochord; ng, neural groove;
nt, neural tube; s, somite; fp, floor plate; dm, dermomyotome; st, sclerotome; de,

digestive endoderm; rm, renal mesenchyme; rt, renal tubule.

including the floor plate of the neural tube, notochord, dermomyoto-
me of the somite, and cells in the rudiment of the renal tubule and

770