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FLORA DE PABLO Y COLS. AN. R. ACAD. NAC. FARM.

FIGURE 4. Apoptotic cell death in the chick embryo dorsal neural tube. A) Cross-
section of the neural tube by scanning electron microscopy of a E1.5 embryo.
B) TUNEL assay detecting apoptotic cells, predominant in the dorsal part (upper) of
the neural tube. C) Dissociated cells from the embryo stained by TUNEL (note bright
apoptotic bodies). D) Mean values of percent of apoptotic cells in E1.5 embryos
treated with antisense oligonucleotides against the insulin receptor (IR oligo AS)

or a control (IR oligo CON).

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