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15PeyroSaintPauL,DebryneDBernardD MockDMDeferGLPharmacokineticsandpharmacodynamicsof MD1003(highdosebiotin)inthetreatmentofprogressivemultiplesclerosisExpertOpinDrugMetabToxicol2016;12327-4416GlickMRRyderKW JacksonSAGraphicalcomparisonsofinterferencesinClinicalChemistryInstrumentationClinChem1986;32(3)470-7517ColonPJGreenDNBiotininterferenceinclinicalimmunoassays JApplLabMed2018941-95118Yang J Wiencek JR Mitigatingbiotininterferencein twoRocheimmunoassaysbypremixingbiotynilatedcapturingmoleculeswithstreptavidincoatedbeadsClinChimActa2020;505130-13519LiDRadulescuAShresthaRRootMKargerAB1KilleenAetalAssociationofBiotinIngestionWithPerformanceofHormoneandNonhormone AssaysinHealthy Adults JAMA2017;318(12)1150-116020MockDM MockNJSerumconcentrationsofbisnorbiotinandbiotinsulfoxideincreaseduringbothacuteandchronicbiotinsupplementationILabClinMed1997;129384-38821PeyroSaintPaulL,DebruyneDBernardDMockDMDeferGLPharmacokineticsandpharmacodynamicsofMD1003(high-dosebiotin)in the treatmentofprogressivemultiple sclerosisExpertOpinDrugMetabolToxicol2016;12(3)327-34422ChunKYBiotininterferenceindiagnostictestsClinChem2017;63619-620246ANALESRANFwwwanalesranfcomInterferenciasporbiotinaenvariosinmunoensayosdeelectroquimioluminiscenciaJoséLuisMartín-CalderónAnRealAcadFarmvol87nº3(2021)·pp239-246AcknowledgmentsAuthorisindebt toDr.LuisCaballero for thelanguagereviewsupport6REFERENCES1RulanderNJCardamoneDSeniorMSnyderPJMasterSRInterference fromanti-streptavidinantibody ArchPatholLab Med2013;1371441-462KatzmanBMLuekeAJDonatoLJJaffeASBaumanNAPrevalenceofbiotin supplementsusageinoutpatientsandplasmabiotinconcentrationinpatientspresentingtotheemergencydepartmentClinBiochem2018;6011-163SamarasingheS MeahF,Singh V,Basit AEmanueleNEmanueleM AMazhari AHolmesEW(2017)biotininterference withroutineclinicalimmunoassaysunderstand thecausesandmitigate therisksEndocrinePracticeAugust2017Vol23No8pp989-9984Zempleni J MockDMBiotinbiochemistryandhumanrequirements,JNutrBiochem(1999);10(3)128–1385OttenJJHellwigJP,MeyersLDDietaryReferenceIntakesTheEssentialGuide toNutrientRequirements,National AcademiesPress,20066J Zempleni YIHassanSS WijeratneBiotinandbiotinidasedeficiency,ExpRevEndocrinolMetab(2008);3(6)715–7247PikettyM-L,PolakMFlectnerIGonzales-BriceñoL,SouberbielleJ-CFalsebiochemicaldiagnosisofhyperthyroidismin streptavidinbiotin-basedimmunoassaystheproblemofbiotinintakeandrelatedinterferencesClinChemLabMed2017;55(6)780-888SedelF,PapeixCBellangerATouitouV,Lebrun-FrenayCGalanaudDetalMulSclerRelatDisord2015;4(2)159-699LiJWagarEMengQHComprehensiveassessmentofbiotininterferenceinimmunoassaysClinChimActa2018;293-29810KummerSHemsenDDistelmeierFBiotintreatmentmimickingGrave´sdiseaseNEngJMed2016;18375(7)704-611BowenRBenavidesRColón-Franco JMKatzmanBM MuthukumarASadrzadehHetalBestpracticesinmitigatingtheriskofbiotininterference withlaboratory testingClinBiochem2019;741-1112Elston MSSehgalSDu ToitS Yarndeley T,Conaglen JVFactitiousGravesdiseaseDue toBiotinImmunoassayInterference-ACaseandReviewoftheLiteratureJClinEndocrinolMetab2016;10113LiDRadulescuAShresthaRT,RootMKargerABKilleenAAetalAssociationofBiotinIngestionWithPerformanceofHormoneandNonhormone AssaysinHealthy Adults JAMA2017;318(12)1150-116014GrimseyP,FreyNBendigG Zizler JLorenzOKasapicDetalPopulationpharmacokineticsofexogenousbiotinandrelationshipbetweenbiotin serumlevelsandin vitroimmunoassayinterferenceIntJPharmacokinet2017;2247-56SideseacitarnuestroartículoBiotininterferenceinseveralelectrochemiluminescenceimmunoassays(eclia)JoséLuisMartín-CalderónAnRealAcadFarmInternetAnRealAcadFarmVol87nº3(2021)·pp239-246DOI1053519/analesranf2021870302