Page 97 - 79_04

Reduction
in
mitochondrial
membrane
peroxidizability
index…

ARTÍCULO

Reduction
in
mitochondrial
membrane
peroxidizability
index

and
protein
lipoxidation
levels
in
the
rat
heart
after
ß-­-
adrenergic
receptor
signaling
interruption
with
the
ß-­-blocker

atenolol

Alexia
Gómez1,
Inés
Sánchez
Román1,
José
Gómez1,
Alba
Naudi2,
Charumati

Pushparaj2,
Manuel
Portero-­-Otin2,
Mónica
López-­-Torres1,
Reinald
Pamplona2,

Gustavo
Barja*1

1Department
of
Animal
Physiology
II,
Faculty
of
Biological
Sciences,
Complutense
University,
Madrid

28040,
Spain
2Department
of
Experimental
Medicine,
Faculty
of
Medicine,
University
of
Lleida-­-IRBLleida,

Lleida
25008,
Spain.

*
Corresponding
author:
Prof.
G.
Barja.
Department
of
Animal
Physiology
II,
Faculty
of
Biological

Sciences,
Complutense
University,
c/Antonio
Novais
nº
2,
Madrid
28040,
Spain.
e-­-mail:

gbarja@bio.ucm.es

Recibido
el
18
de
junio
de
2013
An.
Real
Acad.
Farm.
Vol
79,
Nº
4
(2013),
pag.
613-­-633

ABSTRACT

A
new
mammalian
longevity
model
based
on
ß-­-adrenergic
signaling
interruption

at
the
level
of
adenylyl
cyclase
has
reported
decreased
bone
and
heart
aging
and

mean
and
maximum
longevity
increases
in
AC5KO
mice
(1).
We
decided
to
mimic

this
model
in
male
Wistar
rats
treated
with
the
ß-­-blocker
atenolol
in
the
drinking

water
and
to
check
if
an
oxidative
stress
decrease
could
be
involved.
Atenolol

treatment
did
not
modify
heart
mitROS
generation
rate
and
mitDNA
oxidative

damage
but
significantly
decreased
global
peroxidizability
index
of
mitochondrial

membranes,
as
well
as
protein
lipoxidation,
probably
mediated
by
changes
in

elongases
and
desaturases
activities.

Keywords:
Oxidative
stress;
ß-­-blocker;
Lipid
damage.

RESUMEN

Reducción
en
el
índice
de
peroxidizabilidad
de
la
membrana
mitocondrial
y
niveles
de

lipoxidación
proteícos
en
el
corazón
de
la
rata
después
de
la
interrupción
de
la

señalización
del
receptor
betaadrenérgico
con
el
betabloqueante
atenolol

Un
nuevo
modelo
de
longevidad
en
mamíferos
basado
en
la
interrupción
de
la
vía

de
señalización
beta-­-adrenérgica
a
nivel
de
la
adenilato
ciclasa
ha
revelado
una

613