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Table! I.! 6! Optimal! biotinylated6lectin! concentrations! and! optimal! protein! concentration! range! to!
detect!change!in!several!carbohydrate!structures!of!serum!glycoproteins.!

Abbreviated! Source! Lectin! Protein! Minimum! Maximum!
name! serum! serum!
concentration! concentration! dilution! dilution!
1/6400!
SNA! Sambucus+nigra+ 2! 0.8612.5! 1/1600! 1/102400!
1/6400! 1/25600!
MAL!II! Maackia+amurensis+ 20! 3650! 1/1600! 1/25600!
1/6400! 1/25600!
LEL! Lycopersicon+esculentum+ 20! 3612.5! 1/3200! 1/25600!
1/3200! 1/12800!
WGA! Triticum+vulgare+ 20! 3650! 1/3200! 1/12800!
1/800! 1/12800!
LTL! Lotus+tetragonolobus+ 5! 3612.5! 1/1600! 1/12800!
6! 1/25600!
ECL! Erythrina+cristagalli+ 20! 6625! 6!
6! 6!
EEL! Euonymus+europaeus+ 20! 6625! 6! 6!
6! 6!
DBA! Dolichos+biflorus+ 20! 6625! 6!
6!
UEA!I! Ulex+europaeus+ 20! 66100!

UEA!II! Ulex+europaeus+ 40! 3650!

HPA! Helix+pomatia+ FND! 6!

NPA! Narcissus+pseudonarcissus+ FND! 6!

SucWGA! Triticum+vulgare+ FND! 6!

GSL6I6B4! Bandeiraea+simplicifolia+ FND! 6!

VVA! Vicia+villosa+ FND! 6!

Concentrations!expressed!as!µg/ml.!FND:!Fixation!was!not!detected.!

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Table! II.6! Specific! fixation! capacity! of! biotynylated! lectins! to! ligands! of! pathological! and! control!
sera.!

Abbreviated! LECTIN! Reactivity! RESULTS!
name! Specificity! Comparison!with!controls!

! ! 467!days! 769!months!
Neu5Ac(a266)Gal,!
SNA$ Very!good! 25%!increase! 20%!increase!
Neu5Ac(a266Gal/NAc!
Mal!II! Neu5Ac(a263)Gal! Good! 25%!increase! 15%!increase!

LEL! ! Good! 20%!increase! 6%!increase!
(GlcNAc)3,!(GlcNAc)4!
WAG! Good! 12%!increase! 10%!increase!
GlcNAc(ß164)3>GlcNAc((ß16
LTL$ 4)2>Neu5Ac! Good! 37%!decrease! No!difference!
ECL! a6L6Fuc! Good! 30%!decrease! 24%!decrease!
EEL! Good! 21%!decrease! 16%!decrease!
DBA! Gal(ß164)GlcNAc! Good! 25%!decrease! 10%!decrease!
UEA!I! Gal(a163)Gal! Weak! No!difference! No!difference!
UEA!II! a6GalNAc! Weak! No!difference! No!difference!
HPA! a6L6Fuc! Very!weak! No!difference! No!difference!
NPA! Very!weak! No!difference! No!difference!
SucWGA! L6Fuc(a162)Gal(ß164)Glc! Very!weak! No!difference! No!difference!
GSL6I6B4! GalNAc! Very!weak! No!difference! No!difference!
VVA! a6Man! Very!weak! No!difference! No!difference!

GlcNAcNeu5Ac!
a6Gal!

a6GalNAc6Met!>!a6GalNAc6Thr!

124$
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