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An. R. Acad. Nac. Farm., 2010, 76 (3): 343-356
ARTÍCULO
Cytotoxic activity of a-humulene and transcaryo-
phyllene from Salvia officinalis in animal and human
tumor cells
Adil el Hadri1,4*, María Ángeles Gómez del Río1, Jesús Sanz2,
Azucena González Coloma3, Mohamed Idaomar4, Bartolomé Ribas
Ozonas5, Juana Benedí González1, María Isabel Sánchez Reus1
1 Department of Pharmacology, Faculty of Pharmacy, University Complutense
Madrid, 28040-Madrid, Spain.
2 Instituto de Química Orgánica General (CSIC), Juan de la Cierva, 3, 28006-
Madrid, Spain.
3 Centro de Ciencias Medioambientales (CSIC), Serrano, 115, 28006 Madrid,
Spain.
4 Laboratory of Biology and Health, Faculty of Sciences, University Abdelmalek
Essâadi BP 2121, 93002-Tétouan, Morocco.
5 Royal National Academy of Pharmacy, Institute of Spain, Farmacia 11,
28004-Madrid, Spain.
Recibido el 29 de abril de 2010.
ABSTRACT
Background: The purpose of the present work is two-fold: the frac-
tionation of Salvia officinalis essential oil and the cytotoxic study of
this oil with its fractions “in vitro” tumor cell lines. Materials and
Methods: S. officinalis essential oil was obtained by hydrodistillation
and fractionated with column chromatography; the essential oil and
its fractions were analyzed by gas chromatography (GC) coupled to
mass spectrometry (MS). The cytotoxic activity was evaluated in
cellular lines of breast cancer MCF-7, colon cancer HCT-116, and
murine macrophage RAW264.7 cell lines by the MTT assay. Results:
the sub-subfraction F1.1.1 of S. officinalis essential oil containing a-
humulene present highest activity on RAW264.7 and HCT-116 with
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