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ANTONIO L. DOADRIO Y COLS. AN. R. ACAD. NAC. FARM.

Key words: Copper(II); Cefotaxime; Pharmacodynamic;
Pharmacokinetic; HPLC.

RESUMEN

Interacción in vivo del Cu(II) con cefotaxima

Este trabajo estudia la reacción del cobre II con una cefalospo-
rina, la cefotoxima, in vivo; los mecanismos catalíticos que implican
la presencia de complejos ternarios, su farmacocinética y su farma-
codinamia que afectan a la actividad del antibiótico. Las diferencias
más significativas entre las ratas intoxicadas y control fueron obser-
vadas en riñón, pulmón e hígado. También hemos estudiado la ac-
tividad microbicida en Bacillus subtilis CECT 356, Escherichia coli
CECT 434, Escherichia coli CECT 616, Staphilococus aureus spp
aureus CECT 435, Staphilococus aureus spp aureus CECT 239.

Palabras clave: Cobre(II); Cefotaxima; Farmacodinamia; Farma-
cocinética; HPLC.

1. INTRODUCTION

The transition metal interactions to penicillins (1-5) and
cephalosporins (6) were studied previously by spectrophotometric
and potentiometric methods. In the case of cefotaxime, as impurity,
also for HPLC (7).

In those studies it was observed that the effect of Cu(II) on the
penicillins and cephalosporins was to promote their degradation to
coordination complexes of Cu(II) and the corresponding penicilloic
and cephalosporanic acids, (see Figure 1). Other authors have studied
the formation of the complex in vitro between Cu(II) and penicillins
and cephalosporins, by means of the reaction mechanisms,
evaluating the stability constants for the interaction between Cu(II)
and these antibiotics (2, 6).

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