Page 33 - 75_01

VOL. 75 (1), 25-41, 2009 MONO AND BIEXPONENTIAL IN...

z01-ze1 = c+d·q
z01k’1+qe (k’-1-k’1) = e+q·f
z02-ze2 = g+h·q
z02k’2+q? (k’-2-k’2) = j+q·k·0.001

The negative values of f and k indicate that k’1 is greater than k’-1,
and k’2 greater than k’-2. Values of z in the equilibrium depend linear-
ly on q. This suggests that used values of q are in the range of low

concentrations. The apparent rate constant for the process (kf) is lin-

early dependent on the initial concentration of insulin.

5.3. Influence of I (Experiments 4, 8, 9, 10)

The results of experiments 4, 8, 9, 10 are fitted to the equation:

z = a+b·exp(-t·c)+d·exp(-t·e) Eq. 6

Its parameters, coefficient of correlation and sum of squares of
residuals are:

a = 1722, b = 7549, c = 0.1364, d = 2476, e = 0.01455, r = 0.996, s = 2.217·106

The rate constant is independent of the ionic strength. It indicates
that the reaccionantes species do not have electrical charge.

5.4. Influence of ? (Experiments 4, 11, 12, 13)

The results of experiments 4, 11, 12, 13 are fitted to the equation:

z = a/(1+b·?)+c·exp(-t·d/?)+e·exp(-t·f/?) Eq. 7

Its parameters, coefficient of correlation and sum of squares of
residuals are:

a = 1214, b = -0.1093, c = 6595, d = 0.231, e = 3673, f = 0.0235, r =
0.996, s = 2.071·106

35