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VOL. 75 (1), 25-41, 2009 MONO AND BIEXPONENTIAL IN...

3.3. Experimental Procedure

Activity was measured on tubes 1, 7, 13 and 19 at 0 minutes
using a gamma counter. Reaction kinetics were studied by placing
1 mL of the previously mentioned solutions in the plastic coated
tubes and letting them react at different times and at 48 hours, this
being considered infinite time. Each tube was washed to remove
any unbound labeled antibody. Any radioactivity present in the
remaining bound labeled antibody was then measured using a gam-
ma counter.

16 experiments were performed, arranged as follows:

— Experiments 1, 2, 3, 4

Study of the influence of 125I- Insulin concentration (m) upon the
global reaction using tubes 1-28 and solution 10.

— Experiments 4, 5, 6, 7

Study of the influence of Insulin concentration (q) upon the
global reaction using tubes 22-46 and solutions 10,1, 2, 3.

— Experiments 4, 8, 9, 10

Study of the influence of ionic strength (I), using tubes 22-28,
47-64 and solutions 10, 7, 8, 9.

— Experiments 4, 11, 12, 13

Study of the influence of viscosity (?) using tubes 22-28, 65-82 and
solutions 10, 4, 5, 6. The final viscosity of the solutions obtained in
this manner was determined by comparison with a calibration curve
drawn from standard glycerol-water mixes.

— Experiments 4, 14, 15, 16

Study of the influence of temperature (T) using tubes 22-28,
83-100 and solution 10.

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