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DAVID LEÓN NAVARRO Y COLS. AN. R. ACAD. NAC. FARM.
10 µM L-Glu (105 ± 3%, p > 0.05, n = 126 neurons) (Figure 3C). As
it can be observed in Figure 3D no calcium increases in response to
1 µM L-Glu were detected in neither of cells studied (n = 132 somas).
Besides, ATP-mediated responses did not undergo any significant
variations after exposition to 1 µM L-Glu. The pooled average data
compared with the first ATP-induced current were 108 ± 4% (p >
0.05, n = 132 neurons) and 111 ± 4% (p > 0.05, n = 132 neurons).
Figure 3. Cross-talk between ATP and L-Glu responses in somas of 9 div
cultured granule neurons. Effects of several concentrations of L-Glu (100, 10 and
1 µM) on ATP-mediated responses measured in presence (A, C, D) or absence of
Ca2+ (B). ATP-induced currents were studied 30 s and 5 min after L-Glu application
and were compared with the first ATP-response for each neuron. Statistical
significance was calculated by anova test: ***, p < 0.001; **, p < 0.01, significantly
different from first ATP-mediated response.
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