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VICTORIA LÓPEZ-RODAS Y COLS. AN. R. ACAD. NAC. FARM.

Table 1. Toxicity test. Inhibition of photosynthetic performance
(effective quantum yield of photosystem II; FPSII) of species isolated
from the crude oil spill of Arroyo Minero as well as the control isolated
from Doñana National Park, after 48 h. of petroleum exposure
(10% v/v in BG-11 medium)

Inhibition of Inhibition of
photosynthesis by photosynthesis by Fluka
crude oil of Arroyo
Analytical Petroleum
Minero Special Standard

Symploca dubia Undetectable Undetectable

Species from crude oil spill Chlamydomonas Undetectable Undetectable
dinobryonis

Scenedesmus sp Undetectable Undetectable

Scenedesmus Undetectable Undetectable
obtusus

Species from Scenedesmus > 50% > 52%
pristine intermedius

environment

4. DISCUSSION

The crude oil spill of Arroyo Minero is a natural laboratory
to study adaptation of phytoplankton to extreme petroleum
contamination, because at least crude oil spills continuously at least
since 1915. A series of astonishing finding in Arroyo Minero are

892