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VOL. 75 (4), 883-899, 2009 A FASCINATING EXAMPLE OF MICROALGAL ADAPTATION...

Figure 2. a) Symploca dubia. b) Chlamydomonas dinobryonis. c) Scenedesmus sp
(sp nov.?). d) Scenedesmus obtusus.

and Chlamydomonas dinobryonis strains grow more slowly (about
one doubling in three days).

The toxicity test shows that neither crude oil from Arroyo Minero,
nor Fluka Analytical Petroleum Special Standard were toxic for the
species isolated from the crude oil spill of Arroyo Minero (Table 1).
Photosynthesis performance was not inhibited by concentrations of
12% v/v crude oil (11,7% v/v crude oil was maximum dose detected in
Arroyo Minero) after 48 hours under crude oil exposure (a complete
cell cycle in Scenedesmus species). In contrast, both crude oil from
Arroyo Minero as well as Fluka Analytical Petroleum Special Standard
cause inhibition of more than 50% of effective quantum yield of
photosystem II of the control species (Scenedesmus intermedius)
isolated from Doñana National Park, after 48h of 12% v/v petroleum
exposure.

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